PON1 Antibody (OTI2D4)

Western Blot: PON1 Antibody (OTI2D4) [NBP2-03706] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PON1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PON1.

Immunocytochemistry/Immunofluorescence: PON1 Antibody (OTI2D4) [NBP2-03706] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PON1.

Immunohistochemistry-Paraffin: PON1 Antibody (OTI2D4) [NBP2-03706] - Staining of paraffin-embedded Human prostate tissue using anti-PON1 mouse monoclonal antibody.

Flow Cytometry: PON1 Antibody (OTI2D4) [NBP2-03706] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PON1 antibody, and then analyzed by flow cytometry.

Immunohistochemistry-Paraffin: PON1 Antibody (OTI2D4) [NBP2-03706] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PON1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PON1 Antibody (OTI2D4) [NBP2-03706] - Staining of paraffin-embedded Human endometrium tissue using anti-PON1 mouse monoclonal antibody.

Flow Cytometry: PON1 Antibody (OTI2D4) [NBP2-03706] - Analysis of Hela cells, using anti-PON1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry: PON1 Antibody (OTI2D4) [NBP2-03706] - Analysis of Jurkat cells, using anti-PON1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: OTI2D4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.97 mg/ml

PON1 Antibody (OTI2D4) Summary

• Description: Novus Biologicals Mouse PON1 Antibody (OTI2D4) (NBP2-03706) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human PON1 (NP_000437) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PON1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 39.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.97 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PON1 Antibody (OTI2D4)

• A-esterase 1
• Aromatic esterase 1
• arylesterase B-type
• EC 3.1.1.2
• EC 3.1.1.81
• EC 3.1.8.1
• ESA
• ESAesterase A
• K-45
• MVCD5
• paraoxonase 1
• PON 1
• PON1
• PONparaoxonase B-type
• serum aryldiakylphosphatase
• Serum aryldialkylphosphatase 1
• serum paraoxonase/arylesterase 1

Background

The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PON1
• Entrez:
  • 5444(Human)