PON1 Antibody (OTI2D4)

Images

 
Western Blot: PON1 Antibody (2D4) [NBP2-03706] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PON1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunocytochemistry/ Immunofluorescence: PON1 Antibody (2D4) [NBP2-03706] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PON1.
Immunohistochemistry-Paraffin: PON1 Antibody (2D4) [NBP2-03706] - Staining of paraffin-embedded Human prostate tissue using anti-PON1 mouse monoclonal antibody.
Flow Cytometry: PON1 Antibody (2D4) [NBP2-03706] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PON1 antibody, and then analyzed by ...read more
Immunohistochemistry-Paraffin: PON1 Antibody (2D4) [NBP2-03706] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PON1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PON1 Antibody (2D4) [NBP2-03706] - Staining of paraffin-embedded Human endometrium tissue using anti-PON1 mouse monoclonal antibody.
Flow Cytometry: PON1 Antibody (2D4) [NBP2-03706] - Analysis of Hela cells, using anti-PON1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: PON1 Antibody (2D4) [NBP2-03706] - Analysis of Jurkat cells, using anti-PON1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI2D4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

PON1 Antibody (OTI2D4) Summary

Immunogen
Full length human recombinant protein of human PON1 (NP_000437) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PON1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150
Theoretical MW
39.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PON1 Antibody (OTI2D4)

  • A-esterase 1
  • Aromatic esterase 1
  • arylesterase B-type
  • EC 3.1.1.2
  • EC 3.1.1.81
  • EC 3.1.8.1
  • ESA
  • ESAesterase A
  • K-45
  • MVCD5
  • paraoxonase 1
  • PON 1
  • PON1
  • PONparaoxonase B-type
  • serum aryldiakylphosphatase
  • Serum aryldialkylphosphatase 1
  • serum paraoxonase/arylesterase 1

Background

The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PON1 Antibody (NBP2-03706) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-73526AF350
Alexa Fluor 405 NBP2-73526AF405
Alexa Fluor 488 NBP2-73526AF488
Alexa Fluor 532 NBP2-73526AF532
Alexa Fluor 594 NBP2-73526AF594
Alexa Fluor 647 NBP2-73526AF647
Alexa Fluor 700 NBP2-73526AF700
Alexa Fluor 750 NBP2-73526AF750
Allophycocyanin NBP2-73526APC
Allophycocyanin/Cy7 NBP2-73526APCCY7
Biotin NBP2-73526B
DyLight 350 NBP2-73526UV
DyLight 405 NBP2-73526V
DyLight 488 NBP2-73526G
DyLight 550 NBP2-73526R
DyLight 594 NBP2-73526DL594
DyLight 650 NBP2-73526C
DyLight 680 NBP2-73526FR
DyLight 755 NBP2-73526IR
FITC NBP2-73526F
HRP NBP2-73526H
Janelia Fluor 549 NBP2-73526JF549
Janelia Fluor 646 NBP2-73526JF646
PE NBP2-73526PE
PE/Atto594 NBP2-73526PEATT594
PE/Cy5.5 NBP2-73526PECY55
PE/Cy7 NBP2-73526PECY7
PerCP NBP2-73526PCP

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Bioinformatics

Gene Symbol PON1
Entrez