POMC Antibody

Product Discontinued

POMC Antibody Summary

• Immunogen: Antiserum to beta-Lipotropin was raised in rabbits by using N-terminal portion of bovine beta-Lipotropin conjugated to thyroglobulin.
• Specificity: Cross Reactivity: beta-Lipotropin 100%, Met-enkephalin 0.01%, Leu-enkephalin 0.01%, and beta-Endorphin 0.01%.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POMC
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: For slide-mounted tissue sections it is recommended to make working dilution for Immunofluorescence histochemistry is 15 ug/ml, whereas for avidin-biotin Immunohistochemistry antiserum may be diluted to 5 ug/ml.

Reactivity Notes

Human, Mouse and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for POMC Antibody

• ACTH
• adrenocorticotropic hormone
• adrenocorticotropin
• alpha-melanocyte-stimulating hormone
• alpha-MSH
• beta-endorphin
• beta-LPH
• beta-melanocyte-stimulating hormone
• beta-MSH
• CLIP
• corticotropin-like intermediary peptide
• corticotropin-lipotropin
• gamma-LPH
• gamma-MSH
• lipotropin beta
• lipotropin gamma
• LPH
• melanotropin alpha
• melanotropin beta
• melanotropin gamma
• met-enkephalin
• MSH
• NPP
• POC
• POMC
• pro-ACTH-endorphin
• proopiomelanocortin preproprotein
• proopiomelanocortin
• pro-opiomelanocortin

Background

Lipotropin is a hormone produced by the cleavage of pro-opiomelanocortin (POMC). The anterior pituitary gland produces the pro-hormone POMC, which undergoes cleavage to adrenocorticotropin (ACTH) and beta-lipotropin (B-LPH) which is a 90 amino acid polypeptide that is the carboxy-terminal fragment of POMC. It is the predominant opioid peptide of the human pituitary and rat pars distalis and is present in concentrations essentially equimolar with corticotropin.
It stimulates melanocytes to produce melanin, and can also be cleaved into smaller peptides. In humans, gamma-lipotropin, a-MSH, B-MSH, gamma-MSH, a-endorphin, B-endorphin, gamma-endorphin, and met-enkephalin are all possible fragments of B-lipotropin. B-lipotropin also performs lipid-mobilizing functions such as lipolysis and steroidogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POMC