POMC Antibody

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

POMC Antibody Summary

Immunogen
Antiserum to beta-Lipotropin was raised in rabbits by using N-terminal portion of bovine beta-Lipotropin conjugated to thyroglobulin.
Specificity
Cross Reactivity: beta-Lipotropin 100%, Met-enkephalin 0.01%, Leu-enkephalin 0.01%, and beta-Endorphin 0.01%.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POMC
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
Application Notes
For slide-mounted tissue sections it is recommended to make working dilution for Immunofluorescence histochemistry is 15 ug/ml, whereas for avidin-biotin Immunohistochemistry antiserum may be diluted to 5 ug/ml.

Reactivity Notes

Human, Mouse and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for POMC Antibody

  • ACTH
  • adrenocorticotropic hormone
  • adrenocorticotropin
  • alpha-melanocyte-stimulating hormone
  • alpha-MSH
  • beta-endorphin
  • beta-LPH
  • beta-melanocyte-stimulating hormone
  • beta-MSH
  • CLIP
  • corticotropin-like intermediary peptide
  • corticotropin-lipotropin
  • gamma-LPH
  • gamma-MSH
  • lipotropin beta
  • lipotropin gamma
  • LPH
  • melanotropin alpha
  • melanotropin beta
  • melanotropin gamma
  • met-enkephalin
  • MSH
  • NPP
  • POC
  • POMC
  • pro-ACTH-endorphin
  • proopiomelanocortin preproprotein
  • proopiomelanocortin
  • pro-opiomelanocortin

Background

Lipotropin is a hormone produced by the cleavage of pro-opiomelanocortin (POMC). The anterior pituitary gland produces the pro-hormone POMC, which undergoes cleavage to adrenocorticotropin (ACTH) and beta-lipotropin (B-LPH) which is a 90 amino acid polypeptide that is the carboxy-terminal fragment of POMC. It is the predominant opioid peptide of the human pituitary and rat pars distalis and is present in concentrations essentially equimolar with corticotropin.
It stimulates melanocytes to produce melanin, and can also be cleaved into smaller peptides. In humans, gamma-lipotropin, a-MSH, B-MSH, gamma-MSH, a-endorphin, B-endorphin, gamma-endorphin, and met-enkephalin are all possible fragments of B-lipotropin. B-lipotropin also performs lipid-mobilizing functions such as lipolysis and steroidogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol POMC
Entrez