POLR3G Overexpression Lysate

Images

 
Western Blot: POLR3G Overexpression Lysate (Adult Normal) [NBP2-07616] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for POLR3G.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

POLR3G Overexpression Lysate Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_006458. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens POLR3G, mRNA.
Gene
POLR3G

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
25.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for POLR3G Overexpression Lysate

  • DNA-directed RNA polymerase III 32 kDa polypeptide
  • DNA-directed RNA polymerase III subunit G
  • DNA-directed RNA polymerase III subunit RPC7
  • polymerase (RNA) III (DNA directed) polypeptide G (32kD)
  • RNA polymerase III 32 kDa subunit
  • RNA polymerase III subunit C7
  • RPC32RPC7

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

2984-SE
Species: Hu
NB100-1903
Species: Hu, Mu, Rt, Po, Ca, Ha, Pm
Applications: WB, Flow, ICC/IF, IHC-P, IP, B/N
NBP1-84901
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-62690
Species: Hu
Applications: IHC, IHC-P
NB100-2379
Species: Hu, Mu, Rt, Bv, Pm
Applications: WB, Flow, ICC/IF, IHC
NBP1-76929
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-81714
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-80816
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
H00003347-M01
Species: Hu
Applications: WB, ELISA, ICC/IF
NLS1729
Species: Hu, Eq
Applications: WB, IHC, IHC-P
NBP2-59786
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-55224
Species: Hu, Mu, Rt, Po, Bv, Ca, GP
Applications: WB
MAB2018
Species: Hu, Mu, Rt
Applications: WB, Simple Western, CyTOF-reported, ICC, ICFlow
NBP2-67486
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-84597
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-92396
Species: Hu
Applications: IHC, IHC-P
NBP1-82251
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP2-42202
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC
NBP2-07616
Species: Hu
Applications: WB

Publications for POLR3G Lysate (NBP2-07616) (0)

There are no publications for POLR3G Lysate (NBP2-07616).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for POLR3G Lysate (NBP2-07616) (0)

There are no reviews for POLR3G Lysate (NBP2-07616). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for POLR3G Lysate (NBP2-07616) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

NBP2-07616

Additional POLR3G Products

Bioinformatics Tool for POLR3G Lysate (NBP2-07616)

Discover related pathways, diseases and genes to POLR3G Lysate (NBP2-07616). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for POLR3G Lysate (NBP2-07616)

Discover more about diseases related to POLR3G Lysate (NBP2-07616).
 

Pathways for POLR3G Lysate (NBP2-07616)

View related products by pathway.

Blogs on POLR3G

There are no specific blogs for POLR3G, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our POLR3G Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol POLR3G