POLR3D Antibody

Images

 
Western Blot: POLR3D Antibody [NBP1-28737] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-POLR3D ...read more
Immunohistochemistry: POLR3D Antibody [NBP1-28737] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- POLR3D used at a dilution of 1:1,000 (0.2 ug/ml). Detection: DAB

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

POLR3D Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 175 and 225 of human DNA-directed RNA polymerase III subunit D using the numbering given in entry NP_001713.2 (GeneID 661).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POLR3D
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 10 ug/mg
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for POLR3D Antibody

  • BN51 (BHK21) temperature sensitivity complementing
  • BN51
  • BN51TDNA-directed RNA polymerase III 47 kDa polypeptide
  • DNA-directed RNA polymerase III subunit D
  • DNA-directed RNA polymerase III subunit RPC4
  • polymerase (RNA) III (DNA directed) polypeptide D, 44kDa
  • Protein BN51
  • RNA polymerase III 47 kDa subunit
  • RNA polymerase III subunit C4
  • RPC4
  • RPC53 homolog
  • temperature sensitive complementation, cell cycle specific, tsBN51
  • TSBN51RPC53

Background

RNA polymerase III subunit D (POLR3D) is one of 17 subunits of the RNA polymerase III (PolIII) which synthesizes small RNAs, such as 5S rRNA and tRNA. POLR3D include RNA polymerase III subunit C4, RPC4, DNA-directed RNA polymerase III subunit D, DNA-directed RNA polymerase III 47 kDa polypeptide, RPC53, protein BN51, TSBN51, and BN51T.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol POLR3D
Entrez
Uniprot