POLR3A Antibody

Images

 
Western Blot: POLR3A Antibody [NBP1-83204] - Analysis in human cell lines PC-3 and MCF-7 using Anti-POLR3A antibody. Corresponding POLR3A RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.
Immunocytochemistry/ Immunofluorescence: POLR3A Antibody [NBP1-83204] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: POLR3A Antibody [NBP1-83204] - Staining of human colon shows strong cytoplasmic and nuclear positivity in glandular cells.
Western Blot: POLR3A Antibody [NBP1-83204] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line PC-3

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
       

Orthogonal Strategies

 

Order Details

POLR3A Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:PSDDNPVRANLRTKGKQYCGKGEDLCANDSYVTIQNSELMSGSMDKGTLGSGSKNNIFYILLRDWGQNEAADAMSRLARLAPVYLSNRGFSI
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Mouse (98%), Rat (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POLR3A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:250 - 1:500
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
POLR3A Recombinant Protein Antigen (NBP1-83204PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for POLR3A Antibody

  • DNA-directed RNA polymerase III largest subunit
  • DNA-directed RNA polymerase III subunit A
  • DNA-directed RNA polymerase III subunit RPC1
  • EC 2.7.7.6
  • hRPC155
  • POL3A
  • polymerase (RNA) III (DNA directed) polypeptide A, 155kDa
  • RNA polymerase III 155 kDa subunit
  • RNA polymerase III subunit C160
  • RNA polymerase III subunit RPC155-D
  • RPC1
  • RPC155RNA polymerase III subunit C1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Diseases for POLR3A Antibody (NBP1-83204)

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Bioinformatics

Gene Symbol POLR3A
Entrez
Uniprot