POLR2C Antibody

Western Blot: POLR2C Antibody [NBP2-19885] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: POLR2C Antibody [NBP2-19885] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: POLR2C protein stained by POLR2C antibody [N1C3-2] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: POLR2C Antibody [NBP2-19885] - Paraffin-embedded mouse liver. POLR2C antibody [N1C3-2] dilution: 1:500.

Immunohistochemistry-Paraffin: POLR2C Antibody [NBP2-19885] - Paraffin-embedded mouse muscle. POLR2C antibody [N1C3-2] dilution: 1:500.

Immunohistochemistry-Paraffin: POLR2C Antibody [NBP2-19885] - Paraffin-embedded mouse fore brain. POLR2C antibody [N1C3-2] dilution: 1:500.

Immunohistochemistry-Paraffin: POLR2C Antibody [NBP2-19885] - Paraffin-embedded rat middle brain. POLR2C antibody [N1C3-2] dilution: 1:500.

Immunohistochemistry-Paraffin: POLR2C Antibody [NBP2-19885] - Paraffin-embedded mouse esophagus. POLR2C antibody [N1C3-2] dilution: 1:500.

• Reactivity: Hu, Mu, Rt, Bv, Ch, Mk, Xp, Ze
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

POLR2C Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human POLR2C. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Bovine (100%), Zebrafish (92%), Chicken (94%), Monkey (100%), Xenopus (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POLR2C
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
• Theoretical MW: 31 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rhesus Monkey (100%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for POLR2C Antibody

• DNA-directed RNA polymerase II 33 kDa polypeptide
• DNA-directed RNA polymerase II subunit C
• DNA-directed RNA polymerase II subunit RPB3
• hRPB33
• hsRPB3
• polymerase (RNA) II (DNA directed) polypeptide C (33kD)
• polymerase (RNA) II (DNA directed) polypeptide C, 33kDa
• RNA polymerase II subunit 3
• RNA polymerase II subunit B3
• RPB3
• RPB31
• RPB33

Background

This gene encodes the third largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a cysteine rich region and exists as a heterodimer with another polymerase subunit, POLR2J. These two subunits form a core subassembly unit of the polymerase. A pseudogene has been identified on chromosome 21. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: POLR2C
• Uniprot:
  • P19387(Human)