POLG2 Overexpression Lysate

Images

 
Western Blot: POLG2 Overexpression Lysate (Adult Normal) [NBL1-14572] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for POLG2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

POLG2 Overexpression Lysate Summary

Description
Transient overexpression lysate of polymerase (DNA directed), gamma 2, accessory subunit (POLG2), nuclear gene encoding mitochondrial protein The lysate was created in HEK293T cells, using Plasmid ID RC203462 and based on accession number NM_007215. The protein contains a C-MYC/DDK Tag.
Gene
POLG2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
54.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for POLG2 Overexpression Lysate

  • DNA polymerase gamma accessory 55 kDa subunit
  • DNA polymerase subunit gamma-2, mitochondrial
  • EC 2.7.7.7
  • HP55
  • Mitochondrial DNA polymerase accessory subunit
  • mitochondrial DNA polymerase subunit gamma-2
  • mitochondrial DNA polymerase, accessory subunit
  • MtPolB
  • MTPOLBPOLG-BETA
  • p55
  • PEOA4
  • POLB
  • POLGB
  • PolG-beta
  • polymerase (DNA directed), gamma 2, accessory subunit

Background

The accuracy of mitochondrial DNA (mtDNA) replication depends on the coordinated action of many nuclear-encoded proteins and on the correct balance of nucleotides within the mitochondrial matrix. mtDNA is replicated by DNA polymerase gamma, which is composed of a 140-kD catalytic subunit (POLG1; MIM 174763) and a 55-kD accessory subunit (POLG2).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol POLG2
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