POLG2 Antibody

Western Blot: POLG2 Antibody [H00011232-B01P] - Analysis of POLG2 expression in transfected 293T cell line by POLG2 polyclonal antibody. Lane 1: POLG2 transfected lysate(53.35 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: POLG2 Antibody [H00011232-B01P] - Analysis of purified antibody to POLG2 on HeLa cell. (antibody concentration 10 ug/ml)

Product Discontinued

POLG2 Antibody Summary

• Immunogen: POLG2 (AAH09194.1, 1 a.a. - 485 a.a.) full-length human protein. MRSRVAVRACHKVCRCLLSGFGGRVDAGQPELLTERSSPKGGHVKSHAELEGNGEHPEAPGSGEGSEALLEICQRRHFLSGSKQQLSRDSLLSGCHPGFGPLGVELRKNLAAEWWTSVVVFREQVFPVDALHHKPGPLLPGDSAFRLVSAETLREILQDKELSKEQLVAFLENVLKTSGKLRENLLHGALEHYVNCLDLVNKRLPYGLAQIGVCFHPVFDTKQIRNGVKSIGEKTEASLVWFTPPRTSNQWLDFWLRHRLQWWRKFAMSPSNFSSSDCQDEEGRKGNKLYYNFPWGKELIETLWNLGDHELLHMYPGNVSKLHGRDGRKNVVPCVLSVNGDLDRGMLAYLYDSFQLTENSFTRKKNLHRKVLKLHPCLAPIKVALDVGRGPTLELRQVCQGLFNELLENGISVWPAYLETMQSSLEQLYSKYDEMSILFTVLVTETTLENGLIHLRSRDTTMKEMMHISKLKDFLIKYISSAKNV
• Specificity: POLG2 - polymerase (DNA directed), gamma 2, accessory subunit,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: POLG2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. It has also been used for immunofluorescence.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for POLG2 Antibody

• DNA polymerase gamma accessory 55 kDa subunit
• DNA polymerase subunit gamma-2, mitochondrial
• EC 2.7.7.7
• HP55
• Mitochondrial DNA polymerase accessory subunit
• mitochondrial DNA polymerase subunit gamma-2
• mitochondrial DNA polymerase, accessory subunit
• MtPolB
• MTPOLBPOLG-BETA
• p55
• PEOA4
• POLB
• POLGB
• PolG-beta
• polymerase (DNA directed), gamma 2, accessory subunit

Background

The accuracy of mitochondrial DNA (mtDNA) replication depends on the coordinated action of many nuclear-encoded proteins and on the correct balance of nucleotides within the mitochondrial matrix. mtDNA is replicated by DNA polymerase gamma, which is composed of a 140-kD catalytic subunit (POLG1; MIM 174763) and a 55-kD accessory subunit (POLG2).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLG2
• Entrez:
  • 11232(Human)
• Uniprot:
  • AAH09194.1(Human)