Recombinant Human Podoplanin His Protein

SDS-Page: Recombinant Human Podoplanin Protein [NBC1-18511] - PDPN, 13.4 kDa (130 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human Podoplanin His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 99-207 of Human Podoplanin
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MASTGQPEDD TETTGLEGGV AMPGAEDDVV TPGTSEDRYK SGLTTLVATS VNSVTGIRIE DLPTSESTVH AQEQSPSATA SNVATSHSTE KVDGDTQTTV EKDGLSTVTL

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PDPN
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 13.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 7.5), 0.1 M NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human Podoplanin His Protein

• 36-KD
• Aggrus
• Gp36
• Gp38
• GP40
• HT1A-1
• hT1alpha-1
• hT1alpha-2
• lung type I cell membrane associated glycoprotein
• lung type-I cell membrane-associated glycoprotein (T1A-2)
• OTS 8
• OTS8
• PA2.26 antigen
• PDPN
• Podoplanin
• RANDAM-2
• T1A
• T1A-2
• T1-alpha

Background

Podoplanin(PDPN) is a small mucin-like transmembrane protein, widely expressed in various specialized cell types throughout the body. This is a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. Recombinant human PDPN protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

FAQs for Podoplanin Recombinant Protein (NBC1-18511). (Showing 1 - 1 of 1 FAQ).

1. What is the procedure to denature podoplanin protein?
   • We denature our proteins by diluting them with loading buffer and boiling them at 95 degrees Celsius for 5-10 minutes. If reducing conditions are desired, we add 5% BME or DTT to the loading buffer prior to boiling the sample. With larger proteins (+200kDa) we will heat the samples at 65 degrees Celsius for 10 minutes.

Bioinformatics

• Gene Symbol: PDPN