Podocin/NPHS2 Antibody

Western Blot: Podocin/NPHS2 Antibody [NBP2-26057] - Staining of Human (A) and Mouse (B) Kidney lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.

• Reactivity: Hu, Mu, Po, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Podocin/NPHS2 Antibody Summary

• Description: Novus Biologicals Goat Podocin/NPHS2 Antibody (NBP2-26057) is a polyclonal antibody validated for use in WB and ELISA. Anti-Podocin/NPHS2 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-SPSKPVEPLNPKK corresponding to C-Terminus according to NP_055440.1.
• Predicted Species: Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NPHS2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.5 - 2 ug/mL
• Application Notes: WB: Approx 42-45 kDa band observed in Human and Mouse Kidney lysates (calculated MW of 42.2 kDa according to Human NP_055440.1 and Mouse NP_569723.1)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Podocin/NPHS2 Antibody

• Nephrosis 2
• nephrosis 2, idiopathic, steroid-resistant (podocin)
• NPHS2
• PDCN
• PDCNSRN1podocin
• Podocin
• SRN1

Background

NPHS2 encodes the glomerular protein podocin which plays a role in the regulation of glomerular permeability, and acts as a linker between the plasma membrane and the cytoskeleton. Defects in this gene are the cause of autosomal recessive steroid-resistant nephrotic syndrome (SRN). SRN is characterized by onset between three months and five years, resistance to steroid therapy and rapid progression to end-stage renal disease. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Podocin/NPHS2 Antibody (NBP2-26057)(3)

Publications using NBP2-26057

• Zhang F, Liu J, Yu J et al. Effect of Nephropathy Prescription I on the Expression of Angptl3 and Podocyte-Associated Protein in Mice with Adriamycin-Induced Nephropathy. Evidence-based complementary and alternative medicine : eCAM 2021-04-01 [PMID: 38149181]
• Zhang F, Liu J, Yu J et al. Effect of Nephropathy Prescription I on the Expression of Angptl3 and Podocyte-Associated Protein in Mice with Adriamycin-Induced Nephropathy Evidence-Based Complementary and Alternative Medicine 2022-01-31 [PMID: 36787114] (WB, Mouse)
• Relle M, Cash H, Brochhausen C et al. New perspectives on the renal slit diaphragm protein podocin. Mod Pathol 2011-08-01 [PMID: 21499232]

Bioinformatics

• Gene Symbol: NPHS2
• Entrez:
  • 7827(Human)