PNPT1 Antibody (4C11) - Azide and BSA Free

Western Blot: PNPT1 Antibody (4C11) [NBP2-43725] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PNPase antibody [4C11] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody

Immunocytochemistry/ Immunofluorescence: PNPT1 Antibody (4C11) [NBP2-43725] - PNPT1 antibody [4C11] detects PNPT1 protein at cell junction by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PNPT1 stained by PNPT1 antibody [4C11] (NBP2-43725) diluted at 1:500.Blue: Fluoroshield with DAPI .Scale bar= 10um.

• Reactivity: Hu
• Applications: WB, IP
• Clone: 4C11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PNPT1 Antibody (4C11) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PNPT1 Antibody (4C11) - Azide and BSA Free (NBP2-43725) is a monoclonal antibody validated for use in WB and IP. Anti-PNPT1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen used to generate this antibody corresponds to human PNPT1.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PNPT1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot 1:500-1:3000
• Application Notes: IP-Assay dependent
• Theoretical MW: 86 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for PNPT1 Antibody (4C11) - Azide and BSA Free

• DKFZp762K1914
• EC 2.7.7
• EC 2.7.7.8
• mitochondrial
• OLD35
• old-35
• PNPase 1
• PNPase old-35
• PNPase
• Polynucleotide phosphorylase 1
• Polynucleotide phosphorylase-like protein
• polyribonucleotide nucleotidyltransferase 1

Background

PNPT1 is a subunit of the exosome complex, which is involved in 3-prime-to-5-prime exoribonuclease activity for RNA processing and degradation (Raijmakers et al., 2002 [PubMed 12419256]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PNPT1 Antibody (NBP2-43725)(1)

Publication using NBP2-43725

• Ghosh S, Guimaraes JC, Lanzafame M et al. Prevention of dsRNA-induced interferon signaling by AGO1x is linked to breast cancer cell proliferation EMBO J. 2020-08-19 [PMID: 32812257] (WB, Human)

Bioinformatics

• Gene Symbol: PNPT1