PNPO Antibody (OTI1H9) - Azide and BSA Free

Western Blot: PNPO Antibody (OTI1H9) - Azide and BSA Free [NBP2-73508] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PNPO (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PNPO.

Immunohistochemistry: PNPO Antibody (OTI1H9) - Azide and BSA Free [NBP2-73508] - Staining of paraffin-embedded Human Kidney tissue using anti-PNPO mouse monoclonal antibody.

Immunohistochemistry: PNPO Antibody (OTI1H9) - Azide and BSA Free [NBP2-73508] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PNPO mouse monoclonal antibody.

Immunohistochemistry: PNPO Antibody (OTI1H9) - Azide and BSA Free [NBP2-73508] - Staining of paraffin-embedded Human breast tissue using anti-PNPO mouse monoclonal antibody.

Immunohistochemistry: PNPO Antibody (OTI1H9) - Azide and BSA Free [NBP2-73508] - Staining of paraffin-embedded Human thyroid tissue using anti-PNPO mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI1H9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

PNPO Antibody (OTI1H9) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PNPO Antibody (OTI1H9) - Azide and BSA Free (NBP2-01088) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human PNPO(NP_060599) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PNPO
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Theoretical MW: 29.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for PNPO Antibody (OTI1H9) - Azide and BSA Free

• EC 1.4.3.5
• FLJ10535
• PDXPO
• pyridoxal 5'-phosphate synthase
• pyridoxamine 5'-phosphate oxidase
• Pyridoxamine-phosphate oxidase
• pyridoxine 5'-phosphate oxidase
• pyridoxine-5'-phosphate oxidase

Background

Vitamin B6, or pyridoxal 5-prime-phosphate (PLP), is critical for normal cellular function, and some cancer cells have notable differences in vitamin B6 metabolism compared to their normal counterparts. The rate-limiting enzyme in vitamin B6 synthesis is pyridoxine-5-prime-phosphate (PNP) oxidase (PNPO; EC 1.4.3.5).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PNPO