PNPO Antibody (2C7) - BSA Free

Western Blot: PNPO Antibody (2C7) [NBP1-97941] - The Cell lysates (40ug) were resolved by SDS-PAGE,transferred to PVDF membrane and probed with anti-humanPNPO antibody (1:1000). Proteins were visualized using agoat anti-mouse secondary antibody conjugated to HRP andan ECL detection system.Lane 1.: A549 cell lysateLane 2.: HepG2 cell lysate

Immunocytochemistry/Immunofluorescence: PNPO Antibody (2C7) [NBP1-97941] - Analysis of PNPO in Hep3B cells. The cell was stainedwith ATGA0213 (1:100). The secondary antibody (green) wasused Alexa Fluor 488. DAPI was stained the cell nucleus(blue).

Immunohistochemistry-Paraffin: PNPO Antibody (2C7) [NBP1-97941] - Paraffin embedded sections of human breast cancertissue were incubated with anti-human PNPO (1:150) for2 hours at room temperature. Antigen retrieval wasperformed in 0.1M sodium citrate buffer and detectedusing Diaminobenzidine(DAB).

Flow Cytometry: PNPO Antibody (2C7) [NBP1-97941] - Flow cytometry analysis of PNPO in Jurkat cells. The cell wasstained at 2-5ug for 1x106cells (red). A Goatanti mouse IgG (Alexa fluor 488) was used as the secondaryantibody. Mouse monoclonal IgG was used as the isotypecontrol (dark gray), cells without incubation with primary andsecondary antibody was used as the negative control (lightgray).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 2C7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PNPO Antibody (2C7) - BSA Free Summary

• Immunogen: Recombinant human PNPO (57-261aa) purified from E. coli
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PNPO
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for PNPO Antibody (2C7) - BSA Free

• EC 1.4.3.5
• FLJ10535
• PDXPO
• pyridoxal 5'-phosphate synthase
• pyridoxamine 5'-phosphate oxidase
• Pyridoxamine-phosphate oxidase
• pyridoxine 5'-phosphate oxidase
• pyridoxine-5'-phosphate oxidase

Background

PNPO (pyridoxamine 5'-phosphate oxidase) is a 261 amino acid protein belonging to the pyridoxamine 5'-phosphate oxidase family. It is the rate-limiting enzyme in vitamin B6 synthesis. Vitamin B6 (Pyridoxal 5-prime-phosphate or PLP) is vital for normal cellular function, and some cancer cells have notable differences in vitamin B6 metabolism compared to their normal counterparts. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PNPO
• Uniprot:
  • NP_060599(Human)