PNPO Antibody (2C7)

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Western Blot: PNPO Antibody (AT2C7) [NBP1-97941] The extracts of HepG2(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PNPO (1:500 ~ 1:5000). Proteins were visualized using a ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
2C7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

PNPO Antibody (2C7) Summary

Immunogen
Recombinant human PNPO (57-261aa) purified from E. coli
Specificity
Anti-human PNPO mAb, clone 2C7, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PNPO protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PNPO
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:5000
  • ELISA 1:100-1:2000
Application Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:5000. Recommended starting dilution is 1:1000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for PNPO Antibody (2C7)

  • EC 1.4.3.5
  • FLJ10535
  • PDXPO
  • pyridoxal 5'-phosphate synthase
  • pyridoxamine 5'-phosphate oxidase
  • Pyridoxamine-phosphate oxidase
  • pyridoxine 5'-phosphate oxidase
  • pyridoxine-5'-phosphate oxidase

Background

PNPO (pyridoxamine 5'-phosphate oxidase) is a 261 amino acid protein belonging to the pyridoxamine 5'-phosphate oxidase family. It is the rate-limiting enzyme in vitamin B6 synthesis. Vitamin B6 (Pyridoxal 5-prime-phosphate or PLP) is vital for normal cellular function, and some cancer cells have notable differences in vitamin B6 metabolism compared to their normal counterparts. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PNPO
Uniprot