PMVK/phosphomevalonate kinase Antibody (OTI2F9)

Western Blot: PMVK/phosphomevalonate kinase Antibody (2F9) [NBP2-01157] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PMVK/phosphomevalonate kinase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PMVK/phosphomevalonate kinase.

Immunocytochemistry/Immunofluorescence: PMVK/phosphomevalonate kinase Antibody (2F9) [NBP2-01157] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PMVK/phosphomevalonate kinase.

Flow Cytometry: PMVK/phosphomevalonate kinase Antibody (2F9) [NBP2-01157] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PMVK/phosphomevalonate kinase antibody, and then analyzed by flow cytometry.

• Reactivity: Hu, Rt
• Applications: WB, Flow, ICC/IF
• Clone: OTI2F9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.32 mg/ml

PMVK/phosphomevalonate kinase Antibody (OTI2F9) Summary

• Immunogen: Full length human recombinant protein of human PMVK(NP_006547) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PMVK
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:2000
• Theoretical MW: 21.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.32 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PMVK/phosphomevalonate kinase Antibody (OTI2F9)

• hPMK
• HUMPMKI
• phosphomevalonate kinase
• PMKA
• PMKASE
• PMKEC 2.7.4.2
• PMKI

Background

PMVK (EC 2.7.4.2) is a peroxisomal enzyme that catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate as the fifth reaction of the cholesterol biosynthetic pathway.[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PMVK
• Entrez:
  • 10654(Human)