PMS2 Antibody (PMS2/4373R) - Azide and BSA Free

Immunohistochemistry-Paraffin: PMS2 Antibody (PMS2/4373R) [NBP3-08570] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with PMS2 Recombinant Rabbit Monoclonal Antibody (PMS2/4373R).

Immunohistochemistry-Paraffin: PMS2 Antibody (PMS2/4373R) [NBP3-08570] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with PMS2 Recombinant Rabbit Monoclonal Antibody (PMS2/4373R).

Protein Array: PMS2 Antibody (PMS2/4373R) [NBP3-08570] - Analysis of Protein Array containing more than 19,000 full-length human proteins using PMS2-Monospecific Recombinant Rabbit Monoclonal Antibody (PMS2/4373R).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, MA
• Clone: PMS2/4373R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

PMS2 Antibody (PMS2/4373R) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07615).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to residues within aa1-100 of human PMS2 (exact sequence is proprietary) (Uniprot: P54278 )
• Localization: Nuclear
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PMS2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Protein Array
  • Western Blot 1-2 ug/ml
• Application Notes: Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
• Theoretical MW: 96 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for PMS2 Antibody (PMS2/4373R) - Azide and BSA Free

• DNA mismatch repair protein PMS2
• EC 3.1
• H_DJ0042M02.9
• HNPCC4postmeiotic segregation increased (S. cerevisiae) 2
• mismatch repair endonuclease PMS2
• PMS1 protein homolog 2
• PMS2 postmeiotic segregation increased 2 (S. cerevisiae)
• PMSL2PMS2CL

Background

PMS2 is one of the PMS2 gene family members found in clusters on chromosome 7. The product of this gene is involved in DNA mismatch repair. It forms a heterodimer with MLH1 and this complex interacts with other complexes bound to mismatched bases. Mutations in this gene are associated with hereditary nonpolyposis colorectal cancer, Turcot syndrome, and are a cause of supratentorial primitive neuroectodermal tumors. Alternatively spliced transcript variants have been observed for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PMS2