PMS2 Antibody (4T9W4)

Western Blot: PMS2 Antibody (4T9W4) [NBP3-33352] - Western blot analysis of lysates from Jurkat cells, using [KO Validated] PMS2 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.

Western Blot: PMS2 Antibody (4T9W4) [NBP3-33352] - Western blot analysis of lysates from wild type (WT) and PMS2 knockout (KO) HeLa cells, using [KO Validated] PMS2 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 4T9W4
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

PMS2 Antibody (4T9W4) Summary

• Description: Novus Biologicals Rabbit PMS2 Antibody (4T9W4) (NBP3-33352) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein of human PMS2.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PMS2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 ug/mL
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 96 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for PMS2 Antibody (4T9W4)

• DNA mismatch repair protein PMS2
• EC 3.1
• H_DJ0042M02.9
• HNPCC4postmeiotic segregation increased (S. cerevisiae) 2
• mismatch repair endonuclease PMS2
• PMS1 protein homolog 2
• PMS2 postmeiotic segregation increased 2 (S. cerevisiae)
• PMSL2PMS2CL

Background

PMS2 is one of the PMS2 gene family members found in clusters on chromosome 7. The product of this gene is involved in DNA mismatch repair. It forms a heterodimer with MLH1 and this complex interacts with other complexes bound to mismatched bases. Mutations in this gene are associated with hereditary nonpolyposis colorectal cancer, Turcot syndrome, and are a cause of supratentorial primitive neuroectodermal tumors. Alternatively spliced transcript variants have been observed for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PMS2