PLZF Antibody (6318100) [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HEL 92.1.7 human erythroleukemic cell line, LNCaP human prostate cancer cell line, and 293T human embryonic kidney cell line. PVDF Membrane was probed with 1 µg/mL of Human PLZF Monoclonal Antibody (Catalog # MAB2944) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PLZF at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

PLZF was detected in immersion fixed HL-60 human acute promyelocytic leukemia cell line using Human PLZF Monoclonal Antibody (Catalog # MAB2944) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

HL‑60 human acute promyelocytic leukemia cell line was stained with Human PLZF Monoclonal Antibody (Catalog # MAB2944, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

• Reactivity: Hu
• Applications: WB, CyTOF-ready, Flow, ICC/IF
• Clone: 6318100
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

PLZF Antibody (6318100) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human PLZF
  Met1-Gln254
  Accession # Q05516
• Specificity: Detects human PLZF in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) ZBTB38, rhZNF24, rhZNF143, rhZNF206, rhZNF281, or rhZNF423 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ZBTB16
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PLZF Antibody (6318100) [Unconjugated]

• PLZF
• PLZFzinc finger and BTB domain-containing protein 16
• ZBTB16
• zinc finger and BTB domain containing 16
• zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia)
• Zinc finger protein 145
• Zinc finger protein PLZF
• ZNF145
• ZNF145Promyelocytic leukemia zinc finger protein

Background

Human PLZF is a 74 kDa nuclear protein that belongs to the POK family of transcriptional repressors. It is a 673 amino acid protein that contains an N-terminal BTB domain, followed by an acidic domain, a proline-rich region and a C-terminal zinc-finger domain. PLZF forms homodimers with RAR alpha and LAZ3 with its zinc-finger region. Alternate splice forms exist which are tissue-specific and show a deletion of either the BTB domain, the acidic region, or the proline-rich region. Human PLZF shares 96%, 97%, 96%, and 96% amino acid identity with rat, mouse, canine, and bovine PLZF, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PLZF Antibody (MAB2944)(5)

Publications using MAB2944

• Khampang, S;Lorthongpanich, C;Laowtammathron, C;Klaihmon, P;Meesa, S;Suksomboon, W;Jiamvoraphong, N;Kheolamai, P;Luanpitpong, S;Easley, CA;Mahyari, E;Issaragrisil, S; The dynamic expression of YAP is essential for the development of male germ cells derived from human embryonic stem cells Scientific reports 2024-07-08 [PMID: 38977826] (Immunocytochemistry, Human)
• D Yun, L Zhou, J Shi, X Li, X Wu, F Sun G3BP2, a stress granule assembly factor, is dispensable for spermatogenesis in mice PeerJ, 2022-06-28;10(0):e13532. 2022-06-28 [PMID: 35782098] (IHC, Mouse)
• Hong Wang, Aiping Duan, Jing Zhang, Qi Wang, Yuexian Xing, Zhaohui Qin et al. Glucocorticoid receptor wields chromatin interactions to tune transcription for cytoskeleton stabilization in podocytes Communications Biology 2021-06-03 [PMID: 34083716]
• KW Greeson, KL Fowler, PM Estave, S Kate Thomp, C Wagner, R Clayton Ed, KM Symosko, AN Steves, EM Marder, ML Terrell, H Barton, M Koval, M Marcus, CA Easley Detrimental effects of flame retardant, PBB153, exposure on sperm and future generations Sci Rep, 2020-05-22;10(1):8567. 2020-05-22 [PMID: 32444626] (ICC, Human)
• Hsieh C, Botta G, Gao S, Li T, Van Allen E, Treacy D, Cai C, He H, Sweeney C, Brown M, Balk S, Nelson P, Garraway L, Kantoff P PLZF, a tumor suppressor genetically lost in metastatic castration-resistant prostate cancer, is a mediator of resistance to androgen deprivation therapy. Cancer Res, 2015-03-25;75(10):1944-8. 2015-03-25 [PMID: 25808865] (ChIP, IHC-P, Western Blot, Human)

Bioinformatics

• Gene Symbol: ZBTB16
• Uniprot:
  • Q05516()