PLRG1 Antibody

Images

 
Western Blot: PLRG1 Antibody [NBP1-31265] - Sample (30 ug of whole cell lysate) A: Hep G2 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: PLRG1 Antibody [NBP1-31265] - Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PLRG1 protein stained by PLRG1 antibody C-term diluted at 1:500. ...read more
Immunohistochemistry-Paraffin: PLRG1 Antibody [NBP1-31265] - Paraffin-embedded A549 xenograft, using antibody at 1:100 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Pm, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

PLRG1 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PLRG1. The exact sequence is proprietary.
Localization
Nucleus speckle
Predicted Species
Rat (100%), Porcine (100%), Primate (100%), Xenopus (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PLRG1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PLRG1 Antibody

  • MGC110980
  • pleiotropic regulator 1 (PRL1 homolog, Arabidopsis)
  • PRL1Arabidopsis homolog)

Background

PLRG1 is a spliceosomal protein that is highly conserved across species. PLRG1 has been shown to be part of sub-spliceosomal protein complexes that are essential for pre-mRNA splicing in both yeast and humans. PLRG1 and CDC5L interact directly in vitro. This interaction is mediated by WD40 regions in PLRG1 and the C-terminal domain of CDC5L.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Calculators

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Bioinformatics

Gene Symbol PLRG1