PLRG1 Antibody - BSA Free

Western Blot: PLRG1 Antibody [NBP1-31265] - Sample (30 ug of whole cell lysate) A: Hep G2 7. 5% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry: PLRG1 Antibody [NBP1-31265] - PLRG1 antibody [C3], C-term detects PLRG1 protein at nucleus on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. PLRG1 antibody [C3], C-term (NBP1-31265) dilution: 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

PLRG1 antibody [C3], C-term detects PLRG1 protein at nucleus by immunofluorescent analysis.Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PLRG1 protein stained by PLRG1 antibody [C3], C-term (NBP1-31265) diluted at 1:500.Blue: Hoechst 33342 staining.

• Reactivity: Hu, Mu, Rt, Po, Bv, Pm, Xp
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PLRG1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PLRG1 Antibody - BSA Free (NBP1-31265) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PLRG1. The exact sequence is proprietary.
• Localization: Nucleus speckle
• Predicted Species: Rat (100%), Porcine (100%), Bovine (100%), Xenopus (100%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PLRG1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 57 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for PLRG1 Antibody - BSA Free

• MGC110980
• pleiotropic regulator 1 (PRL1 homolog, Arabidopsis)
• PRL1Arabidopsis homolog)

Background

PLRG1 is a spliceosomal protein that is highly conserved across species. PLRG1 has been shown to be part of sub-spliceosomal protein complexes that are essential for pre-mRNA splicing in both yeast and humans. PLRG1 and CDC5L interact directly in vitro. This interaction is mediated by WD40 regions in PLRG1 and the C-terminal domain of CDC5L.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PLRG1