PlGF-2 Antibody (62503)

• Reactivity: Mu
• Applications: ELISA(Cap)
• Clone: 62503
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

PlGF-2 Antibody (62503) Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse PlGF-2
  Ala24-Pro158
  Accession # P49764
• Specificity: Detects mouse PlGF-2 in ELISAs. In ELISAs, no cross-reactivity or interference was observed with recombinant mouse (rm) VEGF R1, rmVEGF R2, or rmVEGF R3.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Pgf
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Detection: Mouse PlGF-2 Biotinylated Antibody (Catalog number BAF465)
  Standard: Recombinant Mouse PlGF-2 (Catalog number 465-PL)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PlGF-2 Antibody (62503)

• OORS
• PlGF2
• PlGF-2

Background

Placenta growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family of growth factors. PlGF and VEGF share primary structural as well as limited amino acid sequence homology with the A and B chains of PDGF. All eight cysteine residues involved in intra- and inter-chain disulfides are conserved among these growth factors. As a result of alternative splicing, three PlGF RNAs encoding monomeric human PlGF-1, PlGF-2 and PlGF-3 isoform precursors containing 149, 179 and 219 amino acid residues, respectively, have been described. In normal mouse tissues, only one mouse PlGF mRNA encoding the equivalent of human PlGF-2 has been identified. Mouse PlGF-2 encodes a 158 amino acid residue precursor protein. Recombinant mouse PlGF preparations purified from the transfected Sf 21 insect cell conditioned medium contain a mixture of two distinct mature PlGF monomers that are derived from the removal of either a 23 or a 26 residue signal peptide from the amino-terminus of the precursor protein. Mouse PlGF-2 shares 65% amino acid identity with human PlGF-2. The gene for PlGF has been mapped to mouse chromosome 12 and human chromosome 14. PlGF binds with high-affinity to Flt-1, but not to Flk-1/KDR.

1. DiPalma, T. et al. (1996) Mamm. Genome 7:6.
2. Cao, Y. et al. (1997) Biochem. Biophys. Res. Commun. 235:493.
3. Ferrara, N. et al. (1997) Endocrin. Rev. 18:4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Pgf
• Uniprot:
  • P49764()