PlGF-2 Antibody (62503) [Alexa Fluor® 405]

• Reactivity: Mu
• Applications: ELISA(Cap), ELISA(Det)
• Clone: 62503
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 405

PlGF-2 Antibody (62503) [Alexa Fluor® 405] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse PlGF-2
  Ala24-Pro158
  Accession # P49764
• Specificity: Detects mouse PlGF-2 in ELISAs. In ELISAs, no cross-reactivity or interference was observed with recombinant mouse (rm) VEGF R1, rmVEGF R2, or rmVEGF R3.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PlGF-2 Antibody (62503) [Alexa Fluor® 405]

• OORS
• PlGF2
• PlGF-2

Background

Placenta growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family of growth factors. PlGF and VEGF share primary structural as well as limited amino acid sequence homology with the A and B chains of PDGF. All eight cysteine residues involved in intra- and inter-chain disulfides are conserved among these growth factors. As a result of alternative splicing, three PlGF RNAs encoding monomeric human PlGF-1, PlGF-2 and PlGF-3 isoform precursors containing 149, 179 and 219 amino acid residues, respectively, have been described. In normal mouse tissues, only one mouse PlGF mRNA encoding the equivalent of human PlGF-2 has been identified. Mouse PlGF-2 encodes a 158 amino acid residue precursor protein. Recombinant mouse PlGF preparations purified from the transfected Sf 21 insect cell conditioned medium contain a mixture of two distinct mature PlGF monomers that are derived from the removal of either a 23 or a 26 residue signal peptide from the amino-terminus of the precursor protein. Mouse PlGF-2 shares 65% amino acid identity with human PlGF-2. The gene for PlGF has been mapped to mouse chromosome 12 and human chromosome 14. PlGF binds with high-affinity to Flt-1, but not to Flk-1/KDR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.