Plexin B2 Antibody (PSH03-48)

Western Blot: Plexin B2 Antibody (PSH03-48) [NBP3-32828] - Western blot analysis of Plexin B2 on different lysates with Rabbit anti-Plexin B2 antibody (NBP3-32828) at 1/2,000 dilution. Lane 1: LNCaP cell lysate Lane 2: 293T cell lysate Lane 3: HeLa cell lysate Lane 4: U-87 MG cell lysate Lane 5: HepG2 cell lysate Lane 6: K-562 cell lysate Lane 7: Jurkat cell lysate Lane 8: MCF7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 205 kDa Observed band size: 170/240 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32828) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Plexin B2 Antibody (PSH03-48) [NBP3-32828] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Plexin B2 antibody (NBP3-32828) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32828) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: Plexin B2 Antibody (PSH03-48) [NBP3-32828] - Immunocytochemistry analysis of HeLa cells labeling Plexin B2 with Rabbit anti-Plexin B2 antibody (NBP3-32828) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Plexin B2 antibody (NBP3-32828) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: PSH03-48
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Plexin B2 Antibody (PSH03-48) Summary

• Description: Novus Biologicals Rabbit Plexin B2 Antibody (PSH03-48) (NBP3-32828) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within human Plexin B2 aa 1-1,250 / 1,838. (Uniprot: O15031)
• Localization: Cell membrane.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PLXNB2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:2000
  • Western Blot 1:2000
• Theoretical MW: 205 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Plexin B2 Antibody (PSH03-48)

• MM1
• MM1KIAA0315dJ402G11.3
• Nbla00445
• PLEXB2
• Plexin B2
• plexin-B2
• PLXNB2

Background

Plexin B2, also known by its gene name PLXNB2, has 1,838 amino acids and is approximately 205kDa. Plexin B2 is a member of the Plexin family, and localizes to the cell membrane as a transmembrane receptor. Plexin B2 is a receptor for Semaphorins, such as SEMA4C, SEMA4D and SEMA4G, and is implicated in cell migration, cell-cell signaling, and axon guidance. Current research on Plexin B2 is being performed in relation to several diseases and disorders including leukemia, neuronitis and Situs inversus. Plexin B2 has also been shown to have interactions with MET, PFDN1, HMOX2, MST1R, and MLF1IP in the axon guidance pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLXNB2