PLC-gamma 1 [p Tyr783] Antibody (1016D) [Unconjugated]

236-EG) using Rabbit Anti-Human Phospho-PLC- gamma1 (Y783) Polyclonal Antibody (Catalog # MAB74542) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." > 236-EG) using Rabbit Anti-Human Phospho-PLC- gamma1 (Y783) Polyclonal Antibody (Catalog # MAB74542) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." title="PLC- gamma1 phosphorylated at Y783 was detected in immersion fixed A431 human epithelial carcinoma cell line treated with Recombinant Human EGF (Catalog # 236-EG) using Rabbit Anti-Human Phospho-PLC- gamma1 (Y783) Polyclonal Antibody (Catalog # MAB74542) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." />

PLC- gamma1 phosphorylated at Y783 was detected in immersion fixed A431 human epithelial carcinoma cell line treated with Recombinant Human EGF (Catalog # <a class=

Cathelicidin-dependent signaling in platelets. a–i LL-37 induced signaling in isolated human platelets. a–c Flow cytometry analysis of platelet P-selectin surface expression in the presence of a the calcium chelator BAPTA (n = 5) or a phospholipase C inhibitor (U-73122, n = 6), b pertussis or cholera toxin to inhibit G-protein signaling (n = 4), or c inhibitors of tyrosine kinases Src-family kinases (Dasatinib, n = 7) and Syk (R406, n = 5). d Representative western blots of phosphorylated Src-family kinase and phosphorylated Syk upon incubation of platelets with LL-37. Collagen was used as positive control for tyrosine kinase phosphorylation, beta -actin served as loading control. Images are representative of three independent blots. e–i Flow cytometry analysis of LL-37 platelet P-selectin surface expression in the presence of e STAT3 small molecule inhibitor (Stattic, n = 6), f GPVI antibody (HGP5C4, n = 9), g GPIIb/IIIa antibody (Abciximab or Tirofiban, n = 4), h formyl-peptid-receptor (FPR1 or FPR2) antibody, and i inhibitors against the purinergic P2X7-receptor (Boc-MLF 10, WRW4 or A438079, n = 4). j–l CRAMP-induced signaling in isolated mouse platelets. j P-selectin surface expression in the presence of a GPVI depleting antibody (JAQ1, n = 6). k P-selectin surface expression and l phospholipase C phosphorylation in platelet lysates from platelet-specific Syk-deficient mice and respective littermates after stimulation with CRAMP (flow cytometry: n = 10 Syk−/− and n = 5 Syk+/+ animals, western blot analysis n = 5 each). Graphs show mean and SEM. P-values were determined by unpaired (a, f, j–l) or paired (c, e, h) t-test Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29670076), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Applications: ICC/IF
• Clone: 1016D
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

PLC-gamma 1 [p Tyr783] Antibody (1016D) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Phosphopeptide containing the human/mouse/rat PLC-gamma 1 Y783 site.
  Accession # P19174
• Modification: p Tyr783
• Specificity: Detects human, mouse, and rat PLC-gamma 1 when phosphorylated at Y783.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PLCG1
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 3-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: Sodium Azide
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PLC-gamma 1 [p Tyr783] Antibody (1016D) [Unconjugated]

• EC 3.1.4.11
• gamma 1 (formerly subtype 148)
• monophosphatidylinositol phosphodiesterase
• NCKAP3,1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1
• phosphatidylinositol phospholipase C
• phosphoinositidase C
• phosphoinositide phospholipase C
• Phosphoinositide phospholipase C-gamma-1
• phospholipase C, gamma 1
• phospholipase C-148
• Phospholipase C-gamma-1
• Phospholipase C-II
• PLC11-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1
• PLC148
• PLC-148
• PLCG1
• PLCgamma 1
• PLC-gamma 1
• PLC-gamma-1
• PLC-II1-phosphatidyl-D-myo-inositol-4,5-bisphosphate
• PPLCA
• triphosphoinositide phosphodiesterase

Background

Phospholipase C gamma-1 (PLC-gamma 1) is activated by receptor tyrosine kinases in response to growth factors and hormones, and phosphorylation at Tyr783 is a widely used indicator of activation. Activated PLC-gamma 1 catalyzes the hydrolysis of phosphatidylinositol 4,5-biphosphate to produce the second messengers inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). IP3 mobilizes the release of calcium while DAG activates protein kinase C. PLC-gamma 1 plays an important role in regulating cell proliferation and differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PLC-gamma 1 Antibody (MAB74542)(2)

Publications using MAB74542

• Hao, L;Ya, X;Wu, J;Tao, C;Ma, R;Zheng, Z;Mou, S;Ling, Y;Yang, Y;Wang, J;Zhang, Y;Lin, Q;Zhao, J; Somatic PDGFRB activating variants promote smooth muscle cell phenotype modulation in intracranial fusiform aneurysm Journal of biomedical science 2024-05-13 [PMID: 38741091] (Immunohistochemistry, Human)
• J Pircher, T Czermak, A Ehrlich, C Eberle, E Gaitzsch, A Margraf, J Grommes, P Saha, A Titova, H Ishikawa-A, K Stark, T Petzold, T Stocker, LT Weckbach, J Novotny, M Sperandio, B Nieswandt, A Smith, H Mannell, B Walzog, D Horst, O Soehnlein, S Massberg, C Schulz Cathelicidins prime platelets to mediate arterial thrombosis and tissue inflammation Nat Commun, 2018-04-18;9(1):1523. 2018-04-18 [PMID: 29670076]

Bioinformatics

• Gene Symbol: PLCG1
• Uniprot:
  • P19174()