Plastin L Overexpression Lysate (Native) Summary
The lysate was created in HEK293T cells, using plasmid ID RC201670 and based on accession number NM_002298. The protein contains a C-terminal DDK tag.
Homo sapiens lymphocyte cytosolic protein 1 (L-plastin) (LCP1), mRNA.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Lysate Details for Plastin L
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for Plastin L Overexpression Lysate (Native)
- LC64PPLS2bA139H14.1 (lymphocyte cytosolic protein 1 (L-plastin))
- L-plastin (Lymphocyte cytosolic protein 1) (LCP-1) (LC64P)
- lymphocyte cytosolic protein 1 (L-plastin)
- Lymphocyte cytosolic protein 1
- Lymphocyte cytosolic protein-1 (plasmin)
- plastin 2
Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). However, L-plastin has been found in many types of malignant human cells of non-hemopoietic origin suggesting that its expression is induced accompanying tumorigenesis in solid tissues. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
Customers Who Viewed This Item Also Viewed...
Applications: WB, IHC
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, IHC-P
Species: Hu, Mk
Applications: WB, ChIP, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Rb
Applications: WB, IHC
Species: Hu, Mu, Rt
Species: Hu, Mu, Rt(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IP
Applications: WB, Simple Western
Applications: WB, ELISA, IHC, IHC-P, IP
Species: Hu, Mu, Rt, Po, Ch, Ha, Pl, Pm
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu, Rt, Po, Bv
Applications: WB, EM, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, RIA
Species: Hu, Mu, Rt, Bv, Rb
Applications: WB, Flow, GS, IHC, IHC-P, IP
Species: Mu, Rt
Applications: WB, Simple Western, IHC, ICC
Species: Hu, Mu(-)
Applications: WB, ICC/IF, IHC-Fr
Publications for Plastin L Lysate (NBL1-12466) (0)
There are no publications for Plastin L Lysate (NBL1-12466).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for Plastin L Lysate (NBL1-12466) (0)
There are no reviews for Plastin L Lysate (NBL1-12466).
By submitting a review earn points towards our Rewards Program.
- 250 points for product review
- 500 additional points for an image with your product review
- Double points (500) if you are the first to review this product
- Double points (1000) if you are the first to review this product with an image
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for Plastin L Lysate (NBL1-12466) (0)
Additional Plastin L Products
Related Products by Gene
Bioinformatics Tool for Plastin L Lysate (NBL1-12466)
Discover related pathways, diseases and genes to Plastin L Lysate (NBL1-12466). Need help?
Read the Bioinformatics Tool Guide
for instructions on using this tool.
Blogs on Plastin L