Plastin L Overexpression Lysate (Native)

Images

 
Western Blot: L-Plastin Overexpression Lysate (Adult Normal) [NBL1-12466] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for L-Plastin.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Plastin L Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201670 and based on accession number NM_002298. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens lymphocyte cytosolic protein 1 (L-plastin) (LCP1), mRNA.
Gene
LCP1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
70.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Plastin L

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Plastin L Overexpression Lysate (Native)

  • CP64
  • DKFZp781A23186
  • FLJ25423
  • FLJ26114
  • FLJ39956
  • LC64PPLS2bA139H14.1 (lymphocyte cytosolic protein 1 (L-plastin))
  • LCP-1
  • LPL
  • L-plastin (Lymphocyte cytosolic protein 1) (LCP-1) (LC64P)
  • L-PLASTIN
  • lymphocyte cytosolic protein 1 (L-plastin)
  • Lymphocyte cytosolic protein 1
  • Lymphocyte cytosolic protein-1 (plasmin)
  • plastin 2
  • plastin-2

Background

Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). However, L-plastin has been found in many types of malignant human cells of non-hemopoietic origin suggesting that its expression is induced accompanying tumorigenesis in solid tissues. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBL1-12547
Species: Hu
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NBP2-34294
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC-P
NBP2-22106
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-47791
Species: Hu, Mk
Applications: WB, ChIP, ICC/IF, IHC, IHC-P
NBP1-32767
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB300-558
Species: Hu, Rb
Applications: WB, ELISA, ICC/IF, IP
NB110-37253
Species: Hu, Mu, Rt
Applications: WB
NB110-60531
Species: Hu, Mu, Rt(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
AF4497
Species: Hu
Applications: WB, Simple Western
NB600-610
Species: Hu
Applications: WB, ELISA, IHC, IHC-P, IP
NB400-114
Species: Hu, Mu, Rt, Po, Ch, Ha, Pl, Pm
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-52979
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-78159
Species: Hu, Rt, Po, Bv
Applications: WB, EM, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, RIA
NB300-537
Species: Hu, Mu, Rt, Bv, Rb
Applications: WB, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP
AF1443
Species: Mu, Rt
Applications: WB, Simple Western, IHC, ICC
NB400-118
Species: Hu, Mu(-)
Applications: WB, ELISA, ICC/IF

Publications for Plastin L Lysate (NBL1-12466) (0)

There are no publications for Plastin L Lysate (NBL1-12466).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Plastin L Lysate (NBL1-12466) (0)

There are no reviews for Plastin L Lysate (NBL1-12466). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Plastin L Lysate (NBL1-12466) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Plastin L Products

Bioinformatics Tool for Plastin L Lysate (NBL1-12466)

Discover related pathways, diseases and genes to Plastin L Lysate (NBL1-12466). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Plastin L Lysate (NBL1-12466)

Discover more about diseases related to Plastin L Lysate (NBL1-12466).
 

Pathways for Plastin L Lysate (NBL1-12466)

View related products by pathway.

PTMs for Plastin L Lysate (NBL1-12466)

Learn more about PTMs related to Plastin L Lysate (NBL1-12466).

Blogs on Plastin L

There are no specific blogs for Plastin L, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Plastin L Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol LCP1