This antibody is useful in Western blot, not recommended for Immunoprecipitation.
99 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
FAQs for PKN3 Antibody (NBP1-30102). (Showing 1 - 2 of 2 FAQs).
A customer needs an antibody to PKN3 which is specific for this isoform and not cross reacting with the forms PKN1 and PKN2. Would this be suitable for NBP1-30102?
I ran an alignment of the immunogen sequence for NBP1-30102 against the sequences for PKN1 and PKN2 and the similarity is low. We haven't tested for cross reactivity, but the customer should feel confident that within these regions there is not many amino acids shared between PKN3 and PKN1 and PKN2.
What I'm looking for is an antibody that recognizes preferably the N-terminal part of the human and mouse PKN3 kinase (since my protein of interest has been shown to bind to a region just upstream of the kinase domain - between aa 510 and 522). My intention is to use this antibody for Western blotting and IPs. I have found a PKN3 antibody (NBP1-30102) raised against an internal region of the human protein, that can recognize endogenous levels of the protein in HeLa lysates according to your datasheet (Western blot). This antibody however is not recommended for IPs. Does this mean that IPs have been tested and the antibody doesn't work for this application? Since I want to be able to (by Western blotting) detect mouse PKN3, do you know if this antibody is suitable for this?
NBP1-30102 recognizes a region from amino acids 225-275 which would be upstream of the kinase domain. Our antibodies are guaranteed for use in all species and applications listed, so for this antibody, it has been shown to work in Western Blots but looks to be unsuitable for IPs. The IP (-) means that we have tested for its use in IP and have been unsuccessful so we do not recommend if for IP. The region recognized shows only 75% sequence similarity in mouse so it may or may not cross-react with mouse but it is something we cannot guarantee. As far as an alternative antibody, our other PKN3 antibodies we currently sell are raised against full-length PKN3 and are not epitope mapped. One of these could be useful for IP and NBP1-30102 could be used to detect.
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