PKN3 Antibody

Images

 
Western Blot: PKN3 Antibody [NBP1-30102] - Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (T; 50 ug) cells. Antibody used at 0.1 ug/ml.
Western Blot: PKN3 Antibody [NBP1-30102] - Image from verified customer review.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

PKN3 Antibody Summary

Immunogen
maps to a region between residue 225 and 275 of human protein kinase N3 using the numbering given in entry NP_037487.2
Epitope
maps to a region between residue 225 and 275 of human protein kinase N3 using the numbering given in entry NP_037487.2
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PKN3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
Application Notes
This antibody is useful in Western blot, not recommended for Immunoprecipitation.
Theoretical MW
99 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP1-30102 in the following application:

Publications
Read Publications using NBP1-30102.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PKN3 Antibody

  • EC 2.7.11
  • EC 2.7.11.13
  • PKNBETA
  • protein kinase N3
  • Protein kinase PKN-beta
  • Protein-kinase C-related kinase 3
  • RP11-545E17.1
  • serine/threonine-protein kinase N3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PKN3 Antibody (NBP1-30102)(2)

Reviews for PKN3 Antibody (NBP1-30102) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.
We have 2 reviews tested in 1 application: WB.

Reviews using NBP1-30102:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot PKN3 NBP1-30102
Enlarge
reviewed by:
Anonymous
WB Human 06/05/2017
View

Summary

ApplicationWestern Blot
Sample TestedA375m2 cell lysate,Immunoprecipitation
SpeciesHuman
Lot1
Western Blot PKN3 NBP1-30102
Enlarge
reviewed by:
Anonymous
WB Human 08/05/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman
FileView PDF

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for PKN3 Antibody (NBP1-30102). (Showing 1 - 2 of 2 FAQs).

  1. A customer needs an antibody to PKN3 which is specific for this isoform and not cross reacting with the forms PKN1 and PKN2. Would this be suitable for NBP1-30102?
    • I ran an alignment of the immunogen sequence for NBP1-30102 against the sequences for PKN1 and PKN2 and the similarity is low. We haven't tested for cross reactivity, but the customer should feel confident that within these regions there is not many amino acids shared between PKN3 and PKN1 and PKN2.
  2. What I'm looking for is an antibody that recognizes preferably the N-terminal part of the human and mouse PKN3 kinase (since my protein of interest has been shown to bind to a region just upstream of the kinase domain - between aa 510 and 522). My intention is to use this antibody for Western blotting and IPs. I have found a PKN3 antibody (NBP1-30102) raised against an internal region of the human protein, that can recognize endogenous levels of the protein in HeLa lysates according to your datasheet (Western blot). This antibody however is not recommended for IPs. Does this mean that IPs have been tested and the antibody doesn't work for this application? Since I want to be able to (by Western blotting) detect mouse PKN3, do you know if this antibody is suitable for this?
    • NBP1-30102 recognizes a region from amino acids 225-275 which would be upstream of the kinase domain. Our antibodies are guaranteed for use in all species and applications listed, so for this antibody, it has been shown to work in Western Blots but looks to be unsuitable for IPs. The IP (-) means that we have tested for its use in IP and have been unsuccessful so we do not recommend if for IP. The region recognized shows only 75% sequence similarity in mouse so it may or may not cross-react with mouse but it is something we cannot guarantee. As far as an alternative antibody, our other PKN3 antibodies we currently sell are raised against full-length PKN3 and are not epitope mapped. One of these could be useful for IP and NBP1-30102 could be used to detect.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Anonymous
06/05/2017
Application: WB
Species: Human

Anonymous
08/05/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol PKN3
Entrez
Uniprot