PRKCSH Antibody - BSA Free

Western Blot: PRKCSH Antibody [NBP1-32006] - Wild-type (WT) and PRKCSH knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRKCSH antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: PRKCSH Antibody [NBP1-32006] - PRKCSH antibody detects PRKCSH protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PRKCSH protein stained by PRKCSH antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: PRKCSH Antibody [NBP1-32006] - Normal liver.

Western Blot: PRKCSH Antibody [NBP1-32006] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE; antibody diluted at 1:10000.

Immunocytochemistry/Immunofluorescence: PRKCSH Antibody [NBP1-32006] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PRKCSH Antibody - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit PRKCSH Antibody - BSA Free (NBP1-32006) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human PRKCSH. The exact sequence is proprietary.
• Localization: Endoplasmic reticulum
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRKCSH
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:5000-1:20000
• Theoretical MW: 59 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for PRKCSH Antibody - BSA Free

• AGE-R2,80K-H protein
• G19P1AGE-binding receptor 2
• glucosidase 2 subunit beta
• Glucosidase II subunit beta
• glucosidase II, beta subunit
• hepatocystin
• PCLD
• PKCSH
• PLD1
• Protein kinase C substrate 60.1 kDa protein heavy chain
• protein kinase C substrate 80K-H
• protein kinase C substrate, 80 Kda protein

Background

This gene encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum (ER). This protein is an acidic phospho-protein known to be a substrate for protein kinase C. Mutations in this gene have been associated with the autosomal dominant polycystic liver disease (PCLD). Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PRKCSH