PRKCSH Antibody

Images

 
Western Blot: PRKCSH Antibody [NBP1-32006] - (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:10000
Immunocytochemistry/ Immunofluorescence: PRKCSH Antibody [NBP1-32006] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: PRKCSH Antibody [NBP1-32006] - Normal liver.
Western Blot: PRKCSH Antibody [NBP1-32006] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE; antibody diluted at 1:10000.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.94 mg/ml

Order Details

PRKCSH Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PRKCSH. The exact sequence is proprietary.
Localization
Endoplasmic reticulum
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRKCSH
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.94 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PRKCSH Antibody

  • AGE-R2,80K-H protein
  • G19P1AGE-binding receptor 2
  • glucosidase 2 subunit beta
  • Glucosidase II subunit beta
  • glucosidase II, beta subunit
  • hepatocystin
  • PCLD
  • PKCSH
  • PLD1
  • Protein kinase C substrate 60.1 kDa protein heavy chain
  • protein kinase C substrate 80K-H
  • protein kinase C substrate, 80 Kda protein

Background

This gene encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum (ER). This protein is an acidic phospho-protein known to be a substrate for protein kinase C. Mutations in this gene have been associated with the autosomal dominant polycystic liver disease (PCLD). Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PRKCSH