PKN1 Antibody (1B10) - Azide and BSA Free

Western Blot: PKN1 Antibody (1B10) [H00005585-M01] - Analysis of PKN1 expression in transfected 293T cell line by PKN1 monoclonal antibody (M01), clone 1B10.Lane 1: PKN1 transfected lysate(103.9 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: PKN1 Antibody (1B10) [H00005585-M01] - Analysis of monoclonal antibody to PKN1 on HeLa cell . Antibody concentration 10 ug/ml.

Western Blot: PKN1 Antibody (1B10) [H00005585-M01] - Analysis of PKN1 over-expressed 293 cell line, cotransfected with PKN1 Validated Chimera RNAi ( Cat # H00005585-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PKN1 monoclonal antibody (M01) clone 1B10 (Cat # H00005585-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Immunoprecipitation: PKN1 Antibody (1B10) [H00005585-M01] - Analysis of PKN1 transfected lysate using anti-PKN1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PKN1 monoclonal antibody.

ELISA: PKN1 Antibody (1B10) [H00005585-M01] - Detection limit for recombinant GST tagged PKN1 is approximately 3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IP, KD
• Clone: 1B10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PKN1 Antibody (1B10) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PKN1 (AAH40061, 462 a.a. ~ 615 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LDMEPQGCLVAEVTFRNPVIERIPRLRRQKKIFSKQQGKAFQRARQMNIDVATWVRLLRRLIPNATGTGTFSPGASPGSEARTTGDISVEKLNLGTDSDSSPQKSSRDPPSSPSSLSSPIQESTAPELPSETQETPGPALCSPLRKSPLTLEDF
• Specificity: PKN1 - protein kinase N1
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PKN1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PKN1 Antibody (1B10) - Azide and BSA Free

• DBK
• EC 2.7.11
• MGC46204
• PAK1
• PAK1Serine-threonine protein kinase N
• PKN
• PKN1
• PKN-ALPHA
• PKNPKN-ALPHA
• PRK1
• PRK1PAK-1
• PRKCL1
• PRKCL1protein kinase C-related kinase 1
• Protease-activated kinase 1
• Protein kinase C-like 1EC 2.7.11.13
• Protein kinase C-like PKN
• protein kinase N1
• Protein kinase PKN-alpha
• Protein-kinase C-related kinase 1
• serine/threonine protein kinase N
• serine/threonine-protein kinase N1
• serine-threonine kinase N

Background

The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PKN1
• Entrez:
  • 5585(Human)
• OMIM:
  • 601032(Human)
• Uniprot:
  • AAH40061(Human)