PKLR Antibody - BSA Free

Western Blot: PKLR Antibody [NBP2-20027] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKLR Antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: PKLR Antibody [NBP2-20027] - Human breast cancer, using Pyruvate Kinase (liver/RBC) antibody at 1:250 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: PKLR Antibody [NBP2-20027] - A. 20 ug Rat liver lysate/extract 7.5% SDS-PAGE Pyruvate Kinase (liver/RBC) antibody dilution: 1:10000

Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 7.5% SDS PAGE NBP2-20027 diluted at 1:1000

• Reactivity: Hu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PKLR Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PKLR Antibody - BSA Free (NBP2-20027) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human PKLR. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PKLR
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 62 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for PKLR Antibody - BSA Free

• EC 2.7.1.40
• PK1
• PK1PKRL
• PKL
• PKLR
• PKR
• Pyruvate Kinase 1
• pyruvate kinase isozyme R/L
• pyruvate kinase isozymes R/L
• pyruvate kinase type L
• pyruvate kinase, liver and blood cell
• pyruvate kinase, liver and RBC
• Red cell/liver pyruvate kinase
• RPK
• R-type/L-type pyruvate kinase

Background

The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PKLR
• Uniprot:
  • P30613()