PKLR Antibody

Images

 
Western Blot: PKLR Antibody [NBP1-32314] - (30 ug of whole cell lysate) A:Hep G2 7.5% SDS PAGE diluted at 1:500.
Western Blot: PKLR Antibody [NBP1-32314] - Sample (50 ug of whole cell lysate) A: mouse liver 7. 5% SDS PAGE; antibody diluted at 1:500.
Western Blot: PKLR Antibody [NBP1-32314] - 30 ug Raw264. 7 whole cell lysate/extract 7. 5 % SDS-PAGE gel, antibody dilution 1:1000.
Western Blot: PKLR Antibody [NBP1-32314] - A. 30 ug K562 whole cell lysate/extract 7. 5 % SDS-PAGE gel, antibody dilution 1:1000.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.62 mg/ml

Order Details

PKLR Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Pyruvate Kinase (liver/RBC). The exact sequence is proprietary.
Specificity
Pyruvate kinase from liver and RBC.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PKLR
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:20000

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.62 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PKLR Antibody

  • EC 2.7.1.40
  • PK1
  • PK1PKRL
  • PKL
  • PKLR
  • PKR
  • Pyruvate Kinase 1
  • pyruvate kinase isozyme R/L
  • pyruvate kinase isozymes R/L
  • pyruvate kinase type L
  • pyruvate kinase, liver and blood cell
  • pyruvate kinase, liver and RBC
  • Red cell/liver pyruvate kinase
  • RPK
  • R-type/L-type pyruvate kinase

Background

Pyruvate kinase is also known as ATP:pyruvate phosphotransferase. At least three molecular forms with pyruvate kinase activity are known. PK1, the red cell form, is deficient in a type of hemolytic anemia. PK2 is found in kidney. PK3 is found in leukocytes, muscle, platelets, and brain but not in red cells or kidney. PK1 is found also in liver. During fetal development, PK3 changes to PK1 in the liver. PK1 is a tetramer composed of two dissimilar polypeptides of somewhat different molecular weight. It is an allosteric enzyme exhibiting cooperative binding for phosphoenolpyruvate and sensitivity to fructose-1,6-diphosphate. PK3 also is a tetrameric protein but, unlike PK1, all subunits are alike and, not unexpectedly, there is no cooperative behavior. The enzyme is insensitive to fructose-1,6-diphosphate. Patients with deficiency of red cell PK have normal PK2 and PK3. There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. Ab38237 recognizes the M1/M2 isoform of pyruvate kinase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PKLR
Entrez