IFT88 Antibody

Images

 
Western Blot: IFT88 Antibody [NB100-2475] - Staining of A549 (A) cell lysate and Mouse Testes (B) lysate (35 ug protein in RIPA buffer). Antibody at 3 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: IFT88 Antibody [NB100-2475] - Analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 ...read more
Flow Cytometry: IFT88 Antibody [NB100-2475] - Analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

IFT88 Antibody Summary

Immunogen
Peptide with sequence C-KKRIDEDDFADEE corresponding to C-Terminus according to NP_006522.2.
Specificity
This antibody is expected to recognise both reported isoforms (NP_783195.2; NP_006522.2)
Predicted Species
Rat (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IFT88
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/uL
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/Immunofluorescence 10 ug/mL
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Peptide ELISA Detection limit 1:128000
Application Notes
Use in IHC-Frozen reported in scientific literature (PMID: 19208653). WB: Preliminary experiments in human kidney, liver and lung lysates gave no specific signal but low background.
Immunofluorescence: This antibody has been successfully used in IF on Mouse Olfactory and Respiratory Epithelium cells
Publications
Read Publications using
NB100-2475 in the following applications:

Reactivity Notes

ICC/IF successful usage for Mouse nose and lungs is reported in scientific literature (PMID: 19470799).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IFT88 Antibody

  • D13S1056ETPR repeat protein 10
  • DAF19
  • hTg737
  • intraflagellar transport 88 homolog (Chlamydomonas)
  • intraflagellar transport protein 88 homolog
  • MGC26259
  • polaris homolog
  • probe hTg737 (polycystic kidney disease, autosomal recessive)
  • Recessive polycystic kidney disease protein Tg737 homolog
  • tetratricopeptide repeat domain 10
  • Tetratricopeptide repeat protein 10
  • TG737Tg737
  • TTC10

Background

This gene encodes a member of the tetratrico peptide repeat (TPR) family. Mutations of a similar gene in mouse can cause polycystic kidney disease. Two transcript variants encoding distinct isoforms have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IFT88 Antibody (NB100-2475)(4)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IFT88 Antibody (NB100-2475) (0)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol IFT88
Entrez
Uniprot