PKC theta Antibody (453416) [Unconjugated]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, CyTOF-ready, Flow
Clone
453416
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

PKC theta Antibody (453416) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human PKC theta
His580-Ser706
Accession # Q04759
Specificity
Detects human PKC theta. This antibody also detects recombinant human PKC theta, but does not detect any other recombinant human PKC family member.
Details of Functionality
ActivityAssaywCitation not found None None None
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PRKCQ
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • Simple Western 5 ug/mL
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PKC theta Antibody (453416) [Unconjugated]

  • EC 2.7.11
  • EC 2.7.11.13
  • MGC126514
  • MGC141919
  • nPKC-theta
  • PKC theta
  • PRKCQ
  • PRKCT
  • protein kinase C theta type
  • protein kinase C, theta

Background

PKC theta (PRKCQ) is a calcium-independent phospholipid-dependent serine and threonine kinase that belongs to the novel PKC subfamily. PKC theta plays a critical role in several aspects of T cell biology, and knockout mice develop autoimmune disease. PKC theta functions through the activation of NF kappa B and AP1 transcription factors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol PRKCQ
Uniprot