PKC delta Antibody

Western blot shows lysates of LNCaP human prostate cancer cell line and HT-29 human colon adenocarcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human PKCd Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4585) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PKCd at approximately 78 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

PKC delta Antibody Summary

• Immunogen: E. coli-derived recombinant human PKCδ
  His549-Asp676
  Accession # Q05655
• Specificity: Detects human PKCδ in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: PRKCD
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PKC delta Antibody

• EC 2.7.11
• EC 2.7.11.13
• MAY1
• MGC49908
• nPKC-delta
• PKC delta
• PKCD
• PKCy
• PRKCD
• protein kinase C delta type
• protein kinase C delta VIII
• protein kinase C, delta

Background

PKCδ (protein kinase C-delta) is a 78 kDa member of the PKC subfamily, AGC Ser/Thr protein kinase family of enzymes. It is a Ca⁺⁺‑independent and phospholipid‑dependent ubiquitous enzyme that participates in cellular apoptosis. Human PKCδ is 676 amino acids (aa) in length. It contains two general regions: a phosphotyrosine and DAG-type 2 binding region (aa's 1‑90 and 159‑208, respectively), and an ATP-binding catalytic region (aa 349‑603). Functionally, inactive cytoplasmic PKCδ is first activated by Tyr64 and Tyr155 phosphorylation. This induces PKCδ entry into the nucleus, where it is cleaved into 43 kDa and 36 kDa fragments, initiating apoptosis. There are two potential alternate start sites for PKCδ at Met51 and Met74. In some cells, a poorly characterized 160 kDa form of PKCδ has been noted. Over aa 549‑676, human PKCδ is 85% aa identical to mouse PKCδ.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PKC delta Antibody (AF4585)(1)

Publication using AF4585

• Ponassi , Raffaell, Terrinoni , Alessand, Chikh , Anissa, Rufini , Alessand, Lena , Anna Mar, Sayan , Berna S, Melino , Gerry, Candi , Eleonora p63 and p73, members of the p53 gene family, transactivate PKCdelta. Biochem Pharmacol, 2006-09-07;72(11):1417-22. 2006-09-07 [PMID: 16959223] (Western Blot, Human)

Bioinformatics

• Gene Symbol: PRKCD
• Uniprot:
  • Q05655()