PKC delta Antibody (M242)

Western Blot: PKC delta Antibody (M242) [NBP3-23307] - Western blot analysis of adult mouse brain tissue lysate. The blot was probed with mouse monoclonal anti-PKC delta (N-terminal region) at 1:125 (lane 1) and 1:500 (lane 2).

Immunocytochemistry/Immunofluorescence: PKC delta Antibody (M242) [NBP3-23307] - Immunocytochemical labeling of PKC delta in rat PC12 cells differentiated with NGF. The cells were labeled with mouse monoclonal PKC delta (N-terminal region) antibody, then detected using appropriate secondary antibody conjugated to Cy3.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: M242
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

PKC delta Antibody (M242) Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Clone (M242) was generated from a recombinant human protein that included amino acids residues in the N-terminal region. This sequence is conserved in rat and mouse PKC delta, and has low homology to other PKC family members. [UniProt# Q05655]
• Specificity: This antibody detects a 78 kDa protein corresponding to the molecular mass of PKC delta on SDS-PAGE immunoblots of adult mouse brain tissue lysate.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRKCD
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:250
• Theoretical MW: 78 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Alternate Names for PKC delta Antibody (M242)

• EC 2.7.11
• EC 2.7.11.13
• MAY1
• MGC49908
• nPKC-delta
• PKC delta
• PKCD
• PKCy
• PRKCD
• protein kinase C delta type
• protein kinase C delta VIII
• protein kinase C, delta

Background

PKC-delta has a biological activity which is closely related to Pkc1, and PKC-delta activates the Pkc1-mediated pathway through an activation of the Bck1 kinase. PKC-delta appears to play a critical role in growth control of yeast and mammalian cells. Suppression experiments suggest that PKC-delta desensitizes the pathway by regulating an aspect of G protein function (1). PKC-delta phosphorylates hRad9 in vitro and in cells exposed to genotoxic agents. It has been shown that PKC-delta is required for binding of hRad9 to Bcl-2. In concert with these results, inhibition of PKC-delta attenuates Rad9-mediated apoptosis. These findings demonstrate that PKC-delta is responsible for the regulation of Rad9 in the Hus1-Rad1 complex and in the apoptotic response to DNA damage (2). It has been reported a mechanism for the regulation of peripheral B-cell survival by serine/threonine protein kinase Cdelta (PKC-delta): spontaneous death of resting B cells is regulated by nuclear localization of PKC-delta that contributes to phosphorylation of histone H2B at serine 14 (S14-H2B) (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKCD