PKC delta Antibody (2E12) - Azide and BSA Free

Western Blot: PKC delta Antibody (2E12) [H00005580-M06] - Western Blot analysis of PRKCD expression in A-431 ( Cat # L015V1 ).

Immunocytochemistry/Immunofluorescence: PKC delta Antibody (2E12) [H00005580-M06] - Analysis of monoclonal antibody to PRKCD on A-431 cell. Antibody concentration 10 ug/ml

ELISA: PKC delta Antibody (2E12) [H00005580-M06] - Detection limit for recombinant GST tagged PRKCD is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 2E12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PKC delta Antibody (2E12) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PRKCD (NP_006245, 577 a.a. ~ 676 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DILEKLFEREPTKRLGVTGNIKIHPFFKTINWTLLEKRRLEPPFRPKVKSPRDYSNFDQEFLNEKARLSYSDKNLIDSMDQSAFAGFSFVNPKFEHLLED
• Localization: Cytoplasmic
• Specificity: PRKCD - protein kinase C, delta
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRKCD
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PKC delta Antibody (2E12) - Azide and BSA Free

• EC 2.7.11
• EC 2.7.11.13
• MAY1
• MGC49908
• nPKC-delta
• PKC delta
• PKCD
• PKCy
• PRKCD
• protein kinase C delta type
• protein kinase C delta VIII
• protein kinase C, delta

Background

Protein Kinase C delta (PKC delta) is a 78 kDa member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC delta is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of multiple cellular processes including cell cycle progression and apoptosis. Autophosphorylation of serine 664 (serine 662 in mouse and rat) contributes to PKC delta activity. Serum dependent phosphorylation of serine 664 is mediated by mTOR pathway, is protected from dephosphorylation by phosphorylated threonine 505 in the activation loop, and is implicated in prolactin signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PKC delta Antibody (H00005580-M06)(1)

Publication using H00005580-M06

• Chen CJ, Nguyen T, Shively JE. Role of calpain-9 and PKC-delta in the apoptotic mechanism of lumen formation in CEACAM1 transfected breast epithelial cells. Exp Cell Res. 2009-11-01 [PMID: 19909740]

Bioinformatics

• Gene Symbol: PRKCD
• Entrez:
  • 5580(Human)
• OMIM:
  • 176977(Human)
• Uniprot:
  • NP_006245(Human)