Pirh2 Antibody - BSA Free

Western Blot: Pirh2 Antibody [NBP2-19839] - Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Pirh2 Antibody [NBP2-19839] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Pirh2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Pirh2 Antibody - BSA Free (NBP2-19839) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Pirh2. The exact sequence is proprietary.
• Localization: Nucleus; Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RCHY1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Pirh2 Antibody - BSA Free

• Androgen receptor N-terminal-interacting protein
• androgen-receptor N-terminal-interacting protein
• ARNIPRING finger protein 199
• CHIMPRNF199DKFZp586C1620
• CH-rich interacting match with PLAG1
• CH-rich-interacting match with PLAG1
• E3 ubiquitin-protein ligase Pirh2
• EC 6.3.2.-
• hARNIP
• hPirh2
• PIRH2
• PRO1996
• ring finger and CHY zinc finger domain containing 1
• RING finger and CHY zinc finger domain-containing protein 1
• Zinc finger protein 363p53-induced RING-H2 protein
• ZNF363

Background

The protein encoded by this gene has ubiquitin-protein ligase activity. This protein binds with p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. This gene is oncogenic because loss of p53 function contributes directly to malignant tumor development. Transcription of this gene is regulated by p53. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: RCHY1
• Uniprot:
  • Q96PM5(Human)