Pirh2 Antibody

Images

 
Western Blot: Pirh2 Antibody [NBP2-19839] - Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Pirh2 Antibody [NBP2-19839] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.88 mg/ml

Order Details

Pirh2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Pirh2. The exact sequence is proprietary.
Localization
Nucleus; Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RCHY1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.88 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Pirh2 Antibody

  • Androgen receptor N-terminal-interacting protein
  • androgen-receptor N-terminal-interacting protein
  • ARNIPRING finger protein 199
  • CHIMPRNF199DKFZp586C1620
  • CH-rich interacting match with PLAG1
  • CH-rich-interacting match with PLAG1
  • E3 ubiquitin-protein ligase Pirh2
  • EC 6.3.2.-
  • hARNIP
  • hPirh2
  • PIRH2
  • PRO1996
  • ring finger and CHY zinc finger domain containing 1
  • RING finger and CHY zinc finger domain-containing protein 1
  • Zinc finger protein 363p53-induced RING-H2 protein
  • ZNF363

Background

The protein encoded by this gene has ubiquitin-protein ligase activity. This protein binds with p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. This gene is oncogenic because loss of p53 function contributes directly to malignant tumor development. Transcription of this gene is regulated by p53. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RCHY1
Uniprot