PIM2 Antibody

Immunohistochemistry-Paraffin: PIM2 Antibody [NBP2-26114] - 5ug/ml staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunocytochemistry/Immunofluorescence Goat Polyclonal PIM2 Antibody [NBP2-26114] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Flow Cytometry: Goat Polyclonal PIM2 Antibody [NBP2-26114] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PIM2 Antibody Summary

• Description: Novus Biologicals Goat PIM2 Antibody (NBP2-26114) is a polyclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. Anti-PIM2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-QTPAEDVPLNPSK corresponding to C-Terminus according to NP_006866.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PIM2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Detection limit 1:16000
  • Flow Cytometry 10 ug/ml
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunohistochemistry 5 ug/ul
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 0.3-1 ug/ml
• Application Notes: Approx. 30kDa band observed in lysates of cell line K562 and in preliminary testing of cell lines HeLa and Caco-2 and approx. 30-35kDa observed in lysates of cell line HepG2 (calculated MW of 34.2kDa according to NP_006866.2).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PIM2 Antibody

• EC 2.7.11.1
• pim-2 oncogene
• PIM2
• pim-2h
• proto-oncogene Pim-2 (serine threonine kinase)
• serine/threonine protein kinase pim-2
• serine/threonine-protein kinase pim-2

Background

PIM2 belongs to a family of cytoplasmic serine/threonine protein kinases that consists of two other members, PIM1 and PIM3. Expression of this protein is regulated by hematopoietic cytokines. PIM2 is most abundantly expressed in hematopoietic tissues, spleen, thymus, and peripheral blood leukocytes, as well as in testis, small intestine, and colon. It is also highly expressed in human leukemic and lymphoma cell lines. The gene is also expressed in primary spermatocytes. The results suggest a role for PIM2 in proliferating cells as well as during meiosis. PIM2 is thought to be a mediator of p53 mediated apoptosis inhibition by interleukin 3, which supports its role in the formation of various tumours including lymphomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PIM2 Antibody (NBP2-26114)(1)

Publication using NBP2-26114

• Morishita D, Katayama R, Sekimizu K et al. Pim kinases promote cell cycle progression by phosphorylating and down-regulating p27Kip1 at the transcriptional and posttranscriptional levels. Cancer Res 2008-07-01 [PMID: 18593906]

Bioinformatics

• Gene Symbol: PIM2
• Entrez:
  • 11040(Human)