PIM2 Antibody

Images

 
Western Blot: PIM2 Antibody [NBP2-26114] - HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Human PIM2 with C-terminal MYC tag probed with NBP2-26114 (0.1 ug/ml) in Lane A and probed with anti-MYC Tag ...read more
Immunohistochemistry-Paraffin: PIM2 Antibody [NBP2-26114] - 5ug/ml staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

PIM2 Antibody Summary

Immunogen
Peptide with sequence C-QTPAEDVPLNPSK corresponding to C-Terminus according to NP_006866.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PIM2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Immunohistochemistry 0.5-1ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:16000
Application Notes
Western blot: In transfected HEK293 transiently expressing Human PIM2 (myc and DYKDDDDK tagged), a band of approx. 38kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody. Primary incubations were for 1 hour
IHC: Paraffin embedded Human Testis.
Control
PIM2 Knockout HeLa Cell Lysate
Publications
Read Publication using NBP2-26114.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PIM2 Antibody

  • EC 2.7.11.1
  • pim-2 oncogene
  • PIM2
  • pim-2h
  • proto-oncogene Pim-2 (serine threonine kinase)
  • serine/threonine protein kinase pim-2
  • serine/threonine-protein kinase pim-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PIM2 Antibody (NBP2-26114)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol PIM2
Entrez