PIM2 Antibody (OTI5D5)

Images

 
Western Blot: PIM2 Antibody (5D5) [NBP2-02441] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunocytochemistry/ Immunofluorescence: PIM2 Antibody (5D5) [NBP2-02441] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PIM2.
Immunohistochemistry-Paraffin: PIM2 Antibody (5D5) [NBP2-02441] - Staining of paraffin-embedded Human prostate tissue using anti-PIM2 mouse monoclonal antibody.
Flow Cytometry: PIM2 Antibody (5D5) [NBP2-02441] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PIM2 antibody, and then analyzed by ...read more
Immunocytochemistry/ Immunofluorescence: PIM2 Antibody (5D5) [NBP2-02441] - Immunofluorescent staining of HeLa cells using anti-PIM2 mouse monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: PIM2 Antibody (5D5) [NBP2-02441] - Immunofluorescent staining of HT29 cells using anti-PIM2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PIM2 Antibody (5D5) [NBP2-02441] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PIM2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PIM2 Antibody (5D5) [NBP2-02441] - Staining of paraffin-embedded Human Kidney tissue using anti-PIM2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PIM2 Antibody (5D5) [NBP2-02441] - Staining of paraffin-embedded Human liver tissue using anti-PIM2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PIM2 Antibody (5D5) [NBP2-02441] - Staining of paraffin-embedded Human pancreas tissue using anti-PIM2 mouse monoclonal antibody.
Flow Cytometry: PIM2 Antibody (5D5) [NBP2-02441] - Analysis of Jurkat cells, using anti-PIM2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Clone
OTI5D5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

PIM2 Antibody (OTI5D5) Summary

Immunogen
Full length human recombinant protein of human PIM2 (NP_006866) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PIM2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
  • Immunoprecipitation 2ug/500ul
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
PIM2 Knockout HeLa Cell Lysate

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PIM2 Antibody (OTI5D5)

  • EC 2.7.11.1
  • pim-2 oncogene
  • PIM2
  • pim-2h
  • proto-oncogene Pim-2 (serine threonine kinase)
  • serine/threonine protein kinase pim-2
  • serine/threonine-protein kinase pim-2

Background

This gene encodes a protooncogene that acts as a serine/threonine protein kinase. Studies determined the encoded protein functions to prevent apoptosis and to promote cell survival.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PIM2 Antibody (NBP2-02441) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PIM2 Antibody (NBP2-02441). (Showing 1 - 1 of 1 FAQ).

  1. We would like to work with one of your 6 Pim 2 antibodies suitable for flow cytometry. We would like to use it for intracellular staining by FACS analysis. Now we saw that "There are no reviews for PIM2 Antibody (NBP2-02448)(NBP2-02441)(NBP2-02536)(NBP2-02439)(NBP2-02440)(NBP2-02442)." As there are no refferences I would like to know if you could send us a test sample. And please, as there are 6 Pim2 FLOW antibodys, which one would you recomend for us?
    • I recommend NBP2-02536 or NBP2-02441 for Pim2 detection as these have been the most extensively validated in flow cytometry.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-73412AF350
Alexa Fluor 405 NBP2-73412AF405
Alexa Fluor 488 NBP2-73412AF488
Alexa Fluor 532 NBP2-73412AF532
Alexa Fluor 594 NBP2-73412AF594
Alexa Fluor 647 NBP2-73412AF647
Alexa Fluor 700 NBP2-73412AF700
Alexa Fluor 750 NBP2-73412AF750
Allophycocyanin NBP2-73412APC
Allophycocyanin/Cy7 NBP2-73412APCCY7
Biotin NBP2-73412B
DyLight 350 NBP2-73412UV
DyLight 405 NBP2-73412V
DyLight 488 NBP2-73412G
DyLight 550 NBP2-73412R
DyLight 594 NBP2-73412DL594
DyLight 650 NBP2-73412C
DyLight 680 NBP2-73412FR
DyLight 755 NBP2-73412IR
FITC NBP2-73412F
HRP NBP2-73412H
Janelia Fluor 549 NBP2-73412JF549
Janelia Fluor 646 NBP2-73412JF646
PE NBP2-73412PE
PE/Atto594 NBP2-73412PEATT594
PE/Cy5.5 NBP2-73412PECY55
PE/Cy7 NBP2-73412PECY7
PerCP NBP2-73412PCP

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Bioinformatics

Gene Symbol PIM2
Entrez