PILR-alpha Antibody (36H2) - Azide and BSA Free

Western Blot: PILR-alpha Antibody (36H2) [DDX0230P-100] - FDFO3 is a cell-surface monomeric glycoprotein

Flow Cytometry: PILR-alpha Antibody (36H2) [DDX0230P-100] - Surface FACS staining of human PBMC

Flow Cytometry: PILR-alpha Antibody (36H2) [DDX0230P-100] - An Ig-SF inhibitory receptor

• Reactivity: Hu
• Applications: WB, Flow, IP
• Clone: 36H2
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.5 mg/ml

PILR-alpha Antibody (36H2) - Azide and BSA Free Summary

• Immunogen: FDFO3 was used as immunogen.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: PILRA
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris-NaCl (pH 8.0)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Ion exchange chromatography

Notes

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

Alternate Names for PILR-alpha Antibody (36H2) - Azide and BSA Free

• Cell surface receptor FDF03
• FDF03
• inhibitory receptor PILRalpha
• Inhibitory receptor PILR-alpha
• paired immunoglobin-like receptor alpha
• paired immunoglobin-like type 2 receptor alpha
• paired immunoglobulin-like receptor alpha
• paired immunoglobulin-like type 2 receptor alpha
• PILRA
• PILRalpha
• PILR-alpha

Background

Cell signaling pathways rely on a dynamic interaction between activating and inhibiting processes. SHP-1-mediated dephosphorylation of protein tyrosine residues is central to the regulation of several cell signaling pathways. Two types of inhibitory receptor superfamily members are immunoreceptor tyrosine-based inhibitory motif (ITIM)-bearing receptors and their non-ITIM-bearing, activating counterparts. Control of cell signaling via SHP-1 is thought to occur through a balance between PILRalpha-mediated inhibition and PILRbeta-mediated activation. These paired immunoglobulin-like receptor genes are located in a tandem head-to-tail orientation on chromosome 7. This particular gene encodes the ITIM-bearing member of the receptor pair, which functions in the inhibitory role. Alternative splicing has been observed at this locus and three variants, each encoding a distinct isoform, are described. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PILR-alpha Antibody (DDX0230P-100)(4)

Publications using DDX0230P-100

• Clark GJ et al. New insights into the phenotype of human dendritic cell populations. Clin Transl Immunology 2016-01-01 [PMID: 26900474]
• Ohradanova-Revic A et al. Differentiation of human monocytes and derived subsets of macrophages and dendritic cells by the HLDA10 monoclonal antibody panel. Clin Transl Immunology 2016-01-01 [PMID: 26900469]
• Autenrieth SE et al. Profiling of primary peripheral blood- and monocyte-derived dendritic cells using monoclonal antibodies from the HLDA10 Workshop in Wollongong, Australia. Clin Transl Immunology 2015-11-01 [PMID: 26682057]
• Fournier N et al. FDF03, a novel inhibitory receptor of the immunoglobulin superfamily, is expressed by human dendritic and myeloid cells. J Immunol 2000-08-01 [PMID: 10903717]

Bioinformatics

• Gene Symbol: PILRA