pIgR Antibody

Immunohistochemistry-Paraffin: pIgR Antibody [NBP1-32897] - Paraffin-embedded human lung adenocarcinoma. PIGR antibody [N2C1], Internal diluted at 1:500.

Immunohistochemistry-Paraffin: pIgR Antibody [NBP1-32897] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.

HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PIGR stained by PIGR antibody [N2C1], Internal diluted at 1:500.Blue: Fluoroshield with DAPI. Scale bar= 10 μm.

Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PIGR antibody [N2C1], Internal diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

pIgR Antibody Summary

• Description: Novus Biologicals Rabbit pIgR Antibody (NBP1-32897) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human pIgR. The exact sequence is proprietary.
• Localization: Cell membrane, Single-pass type I membrane protein, Secretory component: Secreted
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PIGR
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 83 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for pIgR Antibody

• FLJ22667
• hepatocellular carcinoma associated protein TB6
• Hepatocellular carcinoma-associated protein TB6
• MGC125361
• MGC125362
• pIgR
• Poly-Ig receptor
• polymeric immunoglobulin receptor

Background

The PIGR gene is a member of the immunoglobulin superfamily. The encoded poly-Ig receptor binds polymeric immunoglobulin molecules at the basolateral surface of epithelial cells; the complex is then transported across the cell to be secreted at the apical sur

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PIGR
• Entrez:
  • 5284(Human)
• Uniprot:
  • P01833(Human)