IgA Antibody (7D5F12) - BSA Free

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Western Blot: IgA Antibody (7D5F12) [NBP2-61794] - Analysis using IghA1 mAb against HEK293 (1) and IghA1 (AA: 207-353)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: IgA Antibody (7D5F12) [NBP2-61794] - Analysis of Hela cells using IghA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa ...read more
Immunohistochemistry-Paraffin: IgA Antibody (7D5F12) [NBP2-61794] - Analysis of breast cancer tissues using IghA1 mouse mAb with DAB staining.
Flow Cytometry: IgA Antibody (7D5F12) [NBP2-61794] - Analysis of Hela cells using IghA1 mouse mAb (green) and negative control (red).
Western Blot: IgA Antibody (7D5F12) [NBP2-61794] - Analysis using IghA1 mAb against human IghA1 (AA: 207-353) recombinant protein. (Expected MW is 41.7 kDa)
Western Blot: IgA Antibody (7D5F12) [NBP2-61794] - Analysis using IghA1 mouse mAb against L1210 (1), THP-1 (2), HepG2 (3), and COS7 (4) cell lysate.
ELISA: IgA Antibody (7D5F12) [NBP2-61794] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
7D5F12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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Catalog# & Formulation Size Price

IgA Antibody (7D5F12) - BSA Free Summary

Immunogen
Purified recombinant fragment of human IgA (AA: 207-353) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IGHA1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:50-1:250
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
Theoretical MW
37.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for IgA Antibody (7D5F12) - BSA Free

  • IgA
  • Immunoglobulin A

Background

IGHA1 (Immunoglobulin Heavy Constant Alpha 1) is a Protein Coding gene. Diseases associated with IGHA1 include pseudotumor cerebri. Among its related pathways are Vesicle-mediated transport and Regulation of nuclear SMAD2/3 signaling. GO annotations related to this gene include antigen binding and immunoglobulin receptor binding. An important paralog of this gene is IGHG4.;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Blogs on IgA.


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Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

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Bioinformatics

Gene Symbol IGHA1