pIgR Antibody [Alexa Fluor® 647]

• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 647

pIgR Antibody [Alexa Fluor® 647] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse pIgR
  Lys19-Lys645
  Accession # O70570
• Specificity: Detects mouse pIgR in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human pIgR is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

The mouse polymeric immunoglobulin receptor (plgR; also known as membrane secretory component) is a 115 kDa type I transmembrane glycoprotein that is synthesized as a 771 amino acid (aa) precursor. It includes an 18 aa signal sequence, a 627 aa extracellular domain (ECD) (aa 19‑645), a 23 aa transmembrane segment (aa 646‑668), and a 143 aa cytoplasmic region (aa 669‑771) (1‑3). The ECD consists of five V-type Ig-like domains and a sixth non-Ig domain that connects to the transmembrane region. The ECD of mouse plgR is 65%, 69%, 85%, 62% and 62% aa identical to the equivalent region in human, porcine, rat, bovine and canine, respectively. plgR is expressed on secretory epithelial cells and serves as a carrier that transports IgA and IgM across epithelium (1, 2, 4). On the basolateral surface of epithelial cells, the receptor initially binds non-covalently to IgA via domains #1 and #5 of the plgR. A rearrangement then occurs where a disulfide bond forms between domain #5 of the plgR and an IgA heavy chain (2). This complex is then internalized and transcytosed to the apical surface. A soluble covalent complex called secretory IgA (SIgA) is generated by proteolytic cleavage of the complex in the sixth extracellular domain of plgR and released into the lumen (5). This proteolytically generated plgR fragment is referred to as secretory component (SC). Notably, in human, plgR transcytoses constitutively, with or without ligand, creating both a bound and free, 78 kDa SC following cleavage (3). In mouse, this event would be expected to generate a 95 kDa fragment (1). The receptor component of the complex anchors the SIgA molecule to mucous (6), where it serves to protect mucous membranes that form a barrier between the interior of the body and the external environment (7).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.