PIEZO2 Antibody [Biotin]

• Reactivity: Hu, Mu, Po
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Biotin

PIEZO2 Antibody [Biotin] Summary

• Immunogen: A synthetic peptide made to an internal portion of the human PIEZO2 protein (between residues 1450-1500). [UniProt Q9H5I5]
• Localization: Membrane; Multi-pass membrane protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PIEZO2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined.
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 25385580)

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PIEZO2 Antibody [Biotin]

• C18orf30
• C18orf58
• DA3
• DA5
• DAIPT
• FAM38B
• FAM38B2
• family with sequence similarity 38, member A pseud
• family with sequence similarity 38, member B
• family with sequence similarity 38, member B2
• FLJ23144
• FLJ23403
• FLJ25916
• FLJ34907
• FLJ37734
• FLJ45725
• HsT748
• HsT771
• MWKS
• PIEZO2
• piezo-type mechanosensitive ion channel component
• piezo-type mechanosensitive ion channel component 2
• protein PIEZO2

Background

Piezo2 (FAM38B) is a mechanosensitive ion channel which responds to mechanically activated currents in bladder, colon, lung, and dorsal root ganglia. This suggests Piezo2 is important for somatasensory mechanotransduction including touch and pain. Piezo proteins are fairly novel proteins, and whose sequences are not akin to other ion channels or proteins, and are very large in size.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for PIEZO2 Antibody (NBP1-78538B). (Showing 1 - 4 of 4 FAQ).

1. In my project we need Anti Piezo2 and we find it in your catalogs. PIEZO2 Antibody. I want to know the prediction size of your antibody. The size of the protein is more than 200 kd. but in this photo there there are some sharp band are there the actual bands?
   • The predicted molecular weights for this protein are based on the multiple transcripts associated with this protein. Please see <a href=" http://may2012.archive.ensembl.org/Homo_sapiens/Gene/Summary?g=ENSG00000154864;r=18:10670238-11148761" target="_blank">Ensembl </a> for detailed information. Those expected molecular weights are the following: 318.1, 80.8, 73.8, 62.7 kDa. We see this kind of staining across many of our antibodies for PIEZO2. It would appear that the transcript present is dependent on the tissue type. Based on protein arrays, we do believe that this antibody is specific.
2. Would the immunogen or an antigen be available for this antibody as well?
   • The immunogen is a synthetic peptide made to an internal portion of the human PIEZO2 protein found between amino acids 1450-1500.
3. But the customers question is, if the immunogen would be available to purchase as well, using as control peptide?
   • Unfortunately we do not offer the immunogen commercially for purchase.
4. The customer has some problems concerning the specificity with this antibody and would like to do a preabsorption with the peptide. If the peptide is not available he would need to produce the peptide himself, but needs to know the exact immunogen sequence for this. Could this be provided, please? The customer described as well that there are big problems to publish their experiments when he cannot show that the antibody is specific.
   • Please ask your customer to contact our technical service team at rachel@novusbio.com. The immunogen information is strictly proprietary and cannot be publicly released, however we may discuss further options for the customer.

Bioinformatics

• Gene Symbol: PIEZO2