PIEZO2 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: PIEZO2 Antibody [NBP1-78538] - PIEZO2 antibody was tested at 1:100 in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry: PIEZO2 Antibody [NBP1-78538] - Analysis of PIEZ02 in mouse epidermis using DAB with hematoxylin counterstain.

• Reactivity: Hu, Mu, Po
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PIEZO2 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to an internal portion of the human PIEZO2 protein (between residues 1450-1500). [UniProt Q9H5I5]
• Localization: Membrane; Multi-pass membrane protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PIEZO2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
• Application Notes: In ICC/IF, membrane staining was observed in A431 cells. In IHC-P, membrane and cytoplasmic staining is seen in mouse epidermis tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 25385580)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PIEZO2 Antibody - BSA Free

• C18orf30
• C18orf58
• DA3
• DA5
• DAIPT
• FAM38B
• FAM38B2
• family with sequence similarity 38, member A pseud
• family with sequence similarity 38, member B
• family with sequence similarity 38, member B2
• FLJ23144
• FLJ23403
• FLJ25916
• FLJ34907
• FLJ37734
• FLJ45725
• HsT748
• HsT771
• MWKS
• PIEZO2
• piezo-type mechanosensitive ion channel component
• piezo-type mechanosensitive ion channel component 2
• protein PIEZO2

Background

Piezo2 (FAM38B) is a mechanosensitive ion channel which responds to mechanically activated currents in bladder, colon, lung, and dorsal root ganglia. This suggests Piezo2 is important for somatasensory mechanotransduction including touch and pain. Piezo proteins are fairly novel proteins, and whose sequences are not akin to other ion channels or proteins, and are very large in size.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PIEZO2 Antibody (NBP1-78538)(7)

Publications using NBP1-78538

• N'Guetta PY, McLarnon SR, Tassou A et Al. Comprehensive mapping of sensory and sympathetic innervation of the developing kidney Cell Rep 2024-11-20 [PMID: 39412983]
• Scavuzzo MA, Letai KC, Maeno-Hikichi Y et al. Enteric glial hub cells coordinate intestinal motility bioRxiv : the preprint server for biology 2023-06-09 [PMID: 37333182] (Immunohistochemistry-Paraffin, Porcine)
• Luo Z, Liao X, Luo L Et al. Extracellular ATP and cAMP signaling promote Piezo2-dependent mechanical allodynia after trigeminal nerve compression injury Journal of neurochemistry 2021-11-10 [PMID: 34757653]
• Matsunaga M, Kimura M, Ouchi T et al. Mechanical Stimulation-Induced Calcium Signaling by Piezo1 Channel Activation in Human Odontoblast Reduces Dentin Mineralization Frontiers in Physiology 2021-08-24 [PMID: 34504437] (Immunohistochemistry-Paraffin, Porcine)
• Etem EO, Ceylan GG, Ozaydın S The increased expression of Piezo1 and Piezo2 ion channels in human and mouse bladder carcinoma. Adv Clin Exp Med. [PMID: 30010255] (IHC-P, Human)
• Narayanan P, Sondermann J, Rouwette T et al. Native Piezo2 Interactomics Identifies Pericentrin as a Novel Regulator of Piezo2 in Somatosensory Neurons. J Proteome Res 2016-08-05 [PMID: 27345391]
• Lee W, Leddy Ha, Chen Y et al. Synergy between Piezo1 and Piezo2 channels confers high-strain mechanosensitivity to articular cartilage. Proc. natl. Acad. Sci. U.S.A. 2014-11-25 [PMID: 25385580] (IHC-P, Porcine)

FAQs for PIEZO2 Antibody (NBP1-78538). (Showing 1 - 2 of 2 FAQs).

1. In my project we need Anti Piezo2 and we find it in your catalogs. PIEZO2 Antibody. I want to know the prediction size of your antibody. The size of the protein is more than 200 kd. but in this photo there there are some sharp band are there the actual bands?
   • The predicted molecular weights for this protein are based on the multiple transcripts associated with this protein. Please see <a href=" http://may2012.archive.ensembl.org/Homo_sapiens/Gene/Summary?g=ENSG00000154864;r=18:10670238-11148761" target="_blank">Ensembl </a> for detailed information. Those expected molecular weights are the following: 318.1, 80.8, 73.8, 62.7 kDa. We see this kind of staining across many of our antibodies for PIEZO2. It would appear that the transcript present is dependent on the tissue type. Based on protein arrays, we do believe that this antibody is specific.
2. I am interested in purchasing the PIEZO 1 and PIEZO 2 antibodies and their blocking peptides from Novus. I have read your data sheets that in WBs the bands seen are approximately 286 kDa and 318 kDa, respectively. I would greatly appreciate if you could show me these WBs on human cells For PIEZO1 and 2 and the effects of the blocking peptides (or point me to publications using your Abs).
   • We offer a PIEZO1 blocking peptide specific to product NBP1-78446. We unfortunately do not offer a PIEZO2 blocking peptide. Our western blot data for NBP1-78446, NBP1-78624, and NBP1-78538 was performed by an outside collaborator and they have not given permission to us to release their images. That is why we do not have them available on our website. We will fully guarantee all of our products for any listed application or species. We unfortunately do not have any publications for these products either.

Bioinformatics

• Gene Symbol: PIEZO2
• Entrez:
  • 63895(Human)
• Uniprot:
  • Q9H5I5(Human)