PIEZO2 Antibody [DyLight 650]

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Product Details

Summary
Reactivity Hu, Mu, PoSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 650

PIEZO2 Antibody [DyLight 650] Summary

Immunogen
A synthetic peptide made to an internal portion of the human PIEZO2 protein (between residues 1450-1500) [UniProt Q9H5I5]
Localization
Membrane; Multi-pass membrane protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PIEZO2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This PIEZO2 antibody is useful for Immunocytochemistry/Immunofluorescence, and IHC-paraffin embedded sections. In ICC/IF, membrane staining was observed in A431 cells. In IHC-P, membrane and cytoplasmic staining is seen in mouse epidermis tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reactivity Notes

Human and mouse. Porcine reactivity reported in scientific literature (PMID: 25385580)

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for PIEZO2 Antibody [DyLight 650]

  • C18orf30
  • C18orf58
  • FAM38B
  • FAM38B2
  • family with sequence similarity 38, member B
  • family with sequence similarity 38, member B2
  • FLJ23144
  • FLJ23403
  • FLJ25916
  • FLJ34907
  • FLJ37734
  • FLJ45725
  • HsT748
  • HsT771
  • piezo-type mechanosensitive ion channel component 2
  • protein PIEZO2

Background

Piezo2 (FAM38B) is a mechanosensitive ion channel which responds to mechanically activated currents in bladder, colon, lung, and dorsal root ganglia. This suggests Piezo2 is important for somatasensory mechanotransduction including touch and pain. Piezo proteins are fairly novel proteins, and whose sequences are not akin to other ion channels or proteins, and are very large in size.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for PIEZO2 Antibody (NBP1-78538C). (Showing 1 - 1 of 1 FAQ).

  1. In my project we need Anti Piezo2 and we find it in your catalogs. PIEZO2 Antibody. I want to know the prediction size of your antibody. The size of the protein is more than 200 kd. but in this photo there there are some sharp band are there the actual bands?
    • The predicted molecular weights for this protein are based on the multiple transcripts associated with this protein. Please see <a href=" http://may2012.archive.ensembl.org/Homo_sapiens/Gene/Summary?g=ENSG00000154864;r=18:10670238-11148761" target="_blank">Ensembl </a> for detailed information. Those expected molecular weights are the following: 318.1, 80.8, 73.8, 62.7 kDa. We see this kind of staining across many of our antibodies for PIEZO2. It would appear that the transcript present is dependent on the tissue type. Based on protein arrays, we do believe that this antibody is specific.

Secondary Antibodies

 

Isotype Controls

Additional PIEZO2 Products

PIEZO2 NBP1-78538C

Bioinformatics Tool for PIEZO2 Antibody (NBP1-78538C)

Discover related pathways, diseases and genes to PIEZO2 Antibody (NBP1-78538C). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for PIEZO2 Antibody (NBP1-78538C)

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Research Areas for PIEZO2 Antibody (NBP1-78538C)

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Blogs on PIEZO2.

Touch Infographic: From Touch Receptors to the Brain
The body contains thousands of receptors and nerves which allow us to experience the sense of touch, also referred to as tactile perception. The somatosensory system allows organisms to perceive and decode a wide range of tactile stimuli to allow for...  Read full blog post.

PIEZO1: A Mechanosensitive Ion Channel Protein
PIEZO1, and its close homologue PIEZO2, are mechanosensitive ion channel proteins. Mechanosensitive ion channels couple protein conformation to the mechanics of the surrounding membrane, and switch between a closed state and an open state in response...  Read full blog post.

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Bioinformatics

Gene Symbol PIEZO2