PIAS3 Antibody (4F12)

Images

 
Western Blot: PIAS3 Antibody (4F12) [H00010401-M03] - Analysis of PIAS3 expression in transfected 293T cell line by PIAS3 monoclonal antibody (M03), clone 4F12. Lane 1: PIAS3 transfected lysatE (67 KDa). Lane 2: ...read more
Western Blot: PIAS3 Antibody (4F12) [H00010401-M03] - Western blot analysis of PIAS3 over-expressed 293 cell line, cotransfected with PIAS3 Validated Chimera RNAi or non-transfected control. Blot probed with ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
4F12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

PIAS3 Antibody (4F12) Summary

Immunogen
PIAS3 (NP_006090, 453 a.a. ~ 551 a.a) partial recombinant protein with GST tag.PPTKKHCSVTSAAIPALPGSKGVLTSGHQPSSVLRSPAMGTLGGDFLSSLPLHEYPPAFPLGADIQGLDLFSFLQTESQHYGPSVITSLDEQDALGHF
Specificity
PIAS3 (4F12)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PIAS3
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:100-1:2000
Application Notes
Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validation and ELISA.

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PIAS3 Antibody (4F12)

  • MIZ-type containing 5
  • PIAS3
  • Protein inhibitor of activated STAT protein 3
  • protein inhibitor of activated STAT, 3
  • ZMIZ5

Background

This gene encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for PIAS3 Antibody (H00010401-M03) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PIAS3
Entrez
OMIM
Uniprot