PI 3-Kinase p85 beta Antibody

Western Blot: PI 3-Kinase p85 beta Antibody [NBP1-41066] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-PIK3R2 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. PIK3R2 was also immunoprecipitated by rabbit anti-PIK3R2 antibody NBP1-41067, which recognizes a downstream epitope.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

PI 3-Kinase p85 beta Antibody Summary

• Immunogen: The immunogen for this product maps to a region between residue 75 and 125 of human phosphoinositide-3-kinase, regulatory subunit 2 using the numbering given in entry NP_005018.1 (GeneID 5296).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PIK3R2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunoprecipitation 5-15 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Use in Immunohistochemistry reported in scientific literature (PMID 27096956)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PI 3-Kinase p85 beta Antibody

• P85B
• p85-beta
• phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta
• phosphatidylinositol 3-kinase regulatory subunit beta
• phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta)
• phosphoinositide-3-kinase, regulatory subunit 2 (beta)
• phosphoinositide-3-kinase, regulatory subunit 2 (p85 beta)
• phosphoinositide-3-kinase, regulatory subunit, polypeptide 2 (p85 beta)
• PI 3Kinase p85 beta
• PI 3-Kinase p85 beta
• PI3K regulatory subunit beta
• PI3-kinase regulatory subunit beta
• PI3-kinase subunit p85-beta
• PIK3R2
• ptdIns-3-kinase regulatory subunit beta
• ptdIns-3-kinase regulatory subunit p85-beta

Background

The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 beta). p85 beta is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 beta is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PI 3-Kinase p85 beta Antibody (NBP1-41066)(1)

Publication using NBP1-41066

• Jung CK, Jung SH, Yim SH et al. Predictive microRNAs for lymph node metastasis in endoscopically resectable submucosal colorectal cancer. Oncotarget. 2016-05-31 [PMID: 27096956] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: PIK3R2
• Entrez:
  • 5296(Human)