PI 3-Kinase p85 alpha Antibody (6G10)

Images

 
Western Blot: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Western blot analysis using PI 3 Kinase p85 alpha mAb against human PI 3 Kinase p85 alpha recombinant protein. (Expected MW is 53.4 kDa)
Immunocytochemistry: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Immunofluorescence analysis of HepG2 cells using PI 3 Kinase p85 alpha mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments ...read more
Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PI 3 Kinase p85 alpha mouse mAb with DAB staining.
Flow Cytometry: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Flow cytometric analysis of NIH3T3 cells using PI 3 Kinase p85 alpha mouse mAb (green) and negative control (red).
Immunocytochemistry: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Immunofluorescence analysis of HeLa cells using PI 3 Kinase p85 alpha mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments ...read more
Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using PI 3 Kinase p85 alpha mouse mAb with DAB staining.
ELISA: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready, ICC
Clone
6G10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

PI 3-Kinase p85 alpha Antibody (6G10) Summary

Immunogen
Purified recombinant fragment of human PI 3 Kinase p85 alpha (AA: 159-388) expressed in E. Coli.
Localization
Cytoplasm
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PIK3R1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • CyTOF-ready
  • Immunocytochemistry
Theoretical MW
83.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-22212.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for PI 3-Kinase p85 alpha Antibody (6G10)

  • GRB1
  • GRB1PI3-kinase subunit p85-alpha
  • p85
  • p85-ALPHA
  • Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha
  • phosphatidylinositol 3-kinase regulatory subunit alpha
  • phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)
  • phosphatidylinositol 3-kinase, regulatory, 1
  • phosphatidylinositol 3-kinase-associated p-85 alpha
  • phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
  • PI 3Kinase p85 alpha
  • PI 3-Kinase p85 alpha
  • PI3K regulatory subunit alpha
  • PI3-kinase regulatory subunit alpha
  • PIK3R1
  • PtdIns-3-kinase regulatory subunit alpha
  • PtdIns-3-kinase regulatory subunit p85-alpha

Background

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PI 3-Kinase p85 alpha Antibody (NBP2-22212)(1)

We have publications tested in 1 confirmed species: Human.


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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PIK3R1
Entrez