PI 3-Kinase p110 gamma Antibody

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Western Blot: PI 3-Kinase p110 gamma Antibody [NB100-1097] - Analysis of PI 3 Kinase catalytic subunit gamma in Human Heart lysate using this antibody at 2 ug/ ml. Primary incubated for 1 hour. Detected by western blot ...read more
Western Blot: PI 3-Kinase p110 gamma Antibody [NB100-1097] - (2ug/ml) of Human Heart lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

PI 3-Kinase p110 gamma Antibody Summary

Immunogen
Synthetic peptide VLGIKQGEKHSA, representing the C-Terminus of the human protein (residues 1091-1102), according to NP_002640.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PIK3CG
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Peptide ELISA detection limit 1:2000
Application Notes
Western blot: Approx 110-120kDa band seen in Human Heart lysate (Predicted MW of 121kDa according to NP_002640).
Positive Control
Heart Lysate (NB820-59217)
PI 3-Kinase p110 gamma Lysate (NBL1-14418)
Control Peptide
PI 3-Kinase p110 gamma Peptide (NB100-1097PEP)

Reactivity Notes

Cross reacts with Human. Expected from sequence similarity: Rat, Mouse and Dog.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PI 3-Kinase p110 gamma Antibody

  • 1-phosphatidylinositol 3-kinase
  • EC 2.7.1
  • EC 2.7.1.153
  • p110-gamma
  • p120-PI3K
  • phosphatidylinositol 3 kinase gamma, p110 gamma
  • phosphatidylinositol 3-kinase catalytic 110-kD gamma
  • phosphatidylinositol 3-kinase, catalytic, gamma polypeptide
  • Phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma
  • phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma isoform
  • phosphoinositide-3-kinase gamma catalytic subunit
  • phosphoinositide-3-kinase, catalytic, gamma polypeptide
  • PI 3Kinase p110 gamma
  • PI 3-Kinase p110 gamma
  • PI3CG
  • PI3K
  • PI3K-gamma
  • PI3Kgamma
  • PI3K-gamma
  • PI3-kinase subunit gamma
  • PIK3
  • PIK3CG
  • PtdIns-3-kinase subunit gamma
  • PtdIns-3-kinase subunit p110-gamma

Background

This gene encodes a protein that belongs to the pi3/pi4-kinase family of proteins. The gene product is an enzyme that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. It is an important modulator of extracellular signals, including those elicited by E-cadherin-mediated cell-cell adhesion, which plays an important role in maintenance of the structural and functional integrity of epithelia. In addition to its role in promoting assembly of adherens junctions, the protein is thought to play a pivotal role in the regulation of cytotoxicity in NK cells. The gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PIK3CG
Entrez
Uniprot