PI 3-Kinase p110 delta Antibody (JM83-24)

Western Blot: PI 3-Kinase p110 delta Antibody (JM83-24) [NBP2-67055] - Analysis of PI 3-Kinase p110 delta on rat spleen tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry-Paraffin: PI 3-Kinase p110 delta Antibody (JM83-24) [NBP2-67055] - Analysis of paraffin-embedded human tonsil tissue using anti-PI 3 Kinase p110 delta antibody. Counter stained with hematoxylin.

Western Blot: PI 3-Kinase p110 delta Antibody (JM83-24) [NBP2-67055] - Analysis of PI 3 Kinase p110 delta on mouse spleen tissue lysate using anti-PI 3 Kinase p110 delta antibody at 1/1,000 dilution.

Western Blot: PI 3-Kinase p110 delta Antibody (JM83-24) [NBP2-67055] - Analysis of PI 3-Kinase p110 delta on mouse spleen tissue lysates with Rabbit anti-PI 3-Kinase p110 delta antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 119 kDa Observed band size: 105 kDa. Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry-Paraffin: PI 3-Kinase p110 delta Antibody (JM83-24) [NBP2-67055] - Analysis of paraffin-embedded human spleen tissue using anti-PI 3 Kinase p110 delta antibody. Counter stained with hematoxylin.

Product Discontinued

PI 3-Kinase p110 delta Antibody (JM83-24) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human PI 3-Kinase p110 delta aa 197-246 / 1044. (SwissProt: O00329 Human; SwissProt: O35904 Mouse; SwissProt: D4A5Q1 Rat)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PIK3CD
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PI 3-Kinase p110 delta Antibody (JM83-24)

• EC 2.7.1
• EC 2.7.1.153
• p110D
• P110DELTA
• Phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta
• phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform
• phosphoinositide-3-kinase C
• phosphoinositide-3-kinase, catalytic, delta polypeptide
• PI 3Kinase p110 delta
• PI 3-Kinase p110 delta
• PI3K
• PI3K-delta
• PI3-kinase p110 subunit delta
• PI3-kinase subunit delta
• PIK3CD
• PtdIns-3-kinase subunit delta
• PtdIns-3-kinase subunit p110-delta

Background

PI3-Kinases (PI3-Ks) are a family of lipid kinases that are implicated in signal transduction. PI3-K consists of two subunits; p85 and p110. The p85 subunit localize PI3-K activity to the plasma membrane while the p110 subunit contains the catalytic domain of PI3-K. Four isoforms of p110 has been found; the alpha, beta, gamma, and the delta subunit. The delta isoform is predominantly expressed in leukocytes and has been shown to interact with p85 and GTP-bound Ras via its SH2/SH3 domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PIK3CD