PI 3-Kinase p110 beta/PIK3CB Antibody (SC0580)

Western Blot: PI 3-Kinase p110 beta/PIK3CB Antibody (SC0580) [NBP2-67579] - Western blot analysis of PI 3-Kinase p110 beta/PIK3CB on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for

Immunohistochemistry-Paraffin: PI 3-Kinase p110 beta/PIK3CB Antibody (SC0580) [NBP2-67579] - Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-PI 3-Kinase p110 beta/PIK3CB antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-36, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: PI 3-Kinase p110 beta/PIK3CB Antibody (SC0580) [NBP2-67579] - Flow cytometric analysis of PI 3-Kinase p110 beta/PIK3CB was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).

Western Blot: PI 3-Kinase p110 beta/PIK3CB Antibody (SC0580) [NBP2-67579] - Analysis of PI3-kinase p110 subunit beta on Hela cell lysates using anti-PI3-kinase p110 subunit beta antibody at 1/1,000 dilution.

Flow Cytometry: PI 3-Kinase p110 beta/PIK3CB Antibody (SC0580) [NBP2-67579] - Analysis of Hela cells with PI3-kinase p110 subunit beta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG wa secondary

Product Discontinued

PI 3-Kinase p110 beta/PIK3CB Antibody (SC0580) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human PI 3-Kinase p110 beta/PIK3CB aa 5-52 / 1070. (SwissProt: P42338 Human)
• Localization: Cytoplasm, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PIK3CB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Immunoprecipitation
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PI 3-Kinase p110 beta/PIK3CB Antibody (SC0580)

• P110BETA
• PI 3Kinase p110 beta
• PI 3-Kinase p110 beta
• PI3K
• PI3KBETA
• PIK3C1
• PIK3CB phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit beta
• PIK3CB

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PIK3CB