Phosphoserine phosphatase Antibody (3G12)

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Western Blot: Phosphoserine phosphatase Antibody (3G12) [NBP1-04297] - Cell lysates of Mouse brain (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PSP (1:1000). Proteins were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
3G12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Phosphoserine phosphatase Antibody (3G12) Summary

Immunogen
Recombinant human PSP (1-225aa) purified from E. coli
Specificity
Anti-human PSP mAb, clone 3G12, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PSP protein.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PSPH
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • ELISA 1:100-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Phosphoserine phosphatase Antibody (3G12)

  • L-3-phosphoserine phosphatase
  • O-phosphoserine phosphohydrolase
  • phosphoserine phosphatase
  • PSPase
  • PSPEC 3.1.3.3

Background

Human phosphoserine phosphatase (HPSP), specific for D- and L- phosphoserine, has been identified in all human tissues. HPSP is a Mg(2+)-dependent phosphoserine phosphatase. The three dimensional structure of HPSP reveals the structural and functional role of the divalent cation in the active site of phosphatases. In particular, the complex structures reveal that the open-closed environmental change of the active site, generated by local rearrangement of the alpha-helical bundle domain, is important for the substrate recognition and hydrolysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PSPH
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